| 1. Research Institution | Nagoya University | |
| 2. Research Area | Life Sciences | |
| 3. Research Field | Plant Genetics | |
| 4. Term of Project | FY 2000 - FY 2004 | |
| 5. Project Number | 00L01603 | |
| 6. Title of Project | Novel Gene Function Involved in Higher-Order Regulation of Nutrition-Storage in Plants |
7. Project Leader
| Name | Institution, Department | Title of Position |
|---|---|---|
| Kenzo, Nakamura | Nagoya University, Graduate School of Bioagricultural Sciences | Professor |
8. Core Members
| Names | Institution, Department | Title of Position |
|---|---|---|
| Junji, Yamaguchi | Hokkaido University, Graduate School of Science | Professor |
9. Summary of Research Results
| This research project aimed to identify novel gene function and networks involved in the regulation of nutrient-storage function of plants at an individual level using Arabidopsis and rice. A rice sugar transporter involved in transport of sucrose into developing endosperm was identified and separate roles of ammonium transporters of rice were elucidated. Many novel transcription activators and repressors involved in the sugar-inducible expression of subsets of sink-related genes were identified by screening mutants of sugar-inducible model LUC reporter gene expression, activation tagging, and microarray analysis. A complex gene network that works to achieve high-level expression of seed storage protein genes in developing seeds was elucidated. High-throughput real-time bioluminescence monitoring system was developed and used to screen ~200,000 individuals for circadian clock mutants. Mutants which lost rhythm by a single recessive mutation were isolated and a gene potentially coding for a real clock component was identified. |
10. Key Words
| ( 1 ) Nutrition-storage | ( 2 ) Nutrition transporters | ( 3 ) Sugar-signaling |
| ( 4 ) Novel ranscription factors | ( 5 ) Seed oil | ( 6 ) Storage protein |
| ( 7 ) Circadian clock mutants | ( 8 ) Clock gene | ( 9 ) Vascular system |