Summary of Research Project Results under JSPS FY2004
"Research for the Future Program"

1. Research Institution   Nagoya University
2. Research Area Life Sciences
3. Research Field Plant Genetics
4. Term of Project FY 2000 - FY 2004
5. Project Number 00L01603
6. Title of Project Novel Gene Function Involved in Higher-Order Regulation of Nutrition-Storage in Plants

7. Project Leader

Name Institution, Department Title of Position
Kenzo, Nakamura Nagoya University, Graduate School of Bioagricultural Sciences Professor

8. Core Members

Names Institution, Department Title of Position
Junji, Yamaguchi Hokkaido University, Graduate School of Science Professor

9. Summary of Research Results

This research project aimed to identify novel gene function and networks involved in the regulation of nutrient-storage function of plants at an individual level using Arabidopsis and rice. A rice sugar transporter involved in transport of sucrose into developing endosperm was identified and separate roles of ammonium transporters of rice were elucidated. Many novel transcription activators and repressors involved in the sugar-inducible expression of subsets of sink-related genes were identified by screening mutants of sugar-inducible model LUC reporter gene expression, activation tagging, and microarray analysis. A complex gene network that works to achieve high-level expression of seed storage protein genes in developing seeds was elucidated. High-throughput real-time bioluminescence monitoring system was developed and used to screen ~200,000 individuals for circadian clock mutants. Mutants which lost rhythm by a single recessive mutation were isolated and a gene potentially coding for a real clock component was identified.

10. Key Words

( 1 ) Nutrition-storage ( 2 ) Nutrition transporters ( 3 ) Sugar-signaling
( 4 ) Novel ranscription factors ( 5 ) Seed oil ( 6 ) Storage protein
( 7 ) Circadian clock mutants ( 8 ) Clock gene ( 9 ) Vascular system