Summary of Research Project Results under JSPS FY2003
"Research for the Future Program"

1.Research Institution Hirosaki University
2.Research Area Integrated Fields
3.Research Field Behavior of Substances and Organisms under High Magnetic Field
4.Term of Project FY 1999 - FY 2003
5.Project Number 99I00801
6.Title of Project Biological Responses to Magnetic Fields and the Risk Assessment

7.Project Leader
Name Institution,Department Title of Position
Junji , Miyakoshi Hirosaki University, Faculty of Medicine Professor

8.Core Members

9.Summary of Research Results

   We produced a device to expose cells to the static, low and high frequency magnetic fields. The exposure to the static, low and high frequency magnetic fields had no effect on the basic dynamics of cell growth, life and cycle. The exposure to the static magnetic field alone had no significant effect on cell genotoxicity, however, the combined exposure with radiation and chemicals increased the frequency of mutation and micronucleus formation. In the low frequency magnetic field, the frequency of mutation and chromosomal aberration was increased by exposure to the high-density magnetic field dose-dependently. This result was enhanced by combined exposure with external factors. Molecular biological analysis of mechanism clarified that the low frequency magnetic field had an effect on signaling cascade associated with intracellular potassium and protein kinase. In a study on genetic effects using fruit flies, the strong static magnetic field increased the frequency of chromosomal recombination of a line with gene repair defect although the result was not obtained using a normal line. In medical practice, it was found that the strong static magnetic field had an effect on aligned glioblastoma in a study using collagen. The low frequency magnetic field showed increased insulin secretion of rat islet cells and gene expression.

10.Key Words
(1)magnetic fields   (2)biological effects   (3)cells
(4)Drosophila   (5)genetic toxicity   (6)orientation
(7)medical application   (8)gene expression   (9)germination