Summary of Research Project Results under JSPS FY2002
"Research for the Future Program"

1.Research Institution Tokyo Institute of Technology
2.University-Industry Cooperative Research Committee 167th Committee on Nano-probe Technology
3.Term of Project FY 1999 - FY 2002
4.Project Number 99R16701
5.Title of Project Development of Ultimate Precision Bionanomanipulator

6.Project Leader
Name Institution,Department Title of Position
Atsushi, Ikai Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology Professor

7.Core Members

Name Institution,Department Title of Position
Toshiya, Osada Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology Assistant Professor

8.Summary of Research Results

·1) We have completed the project with high standard results which have been recognized world wide. In nano-mechanics of a single protein molecule, our measurement of the molecular process that leads to the breakdown of its three dimensional strcuture is of exceptionally high quality. Our computer simulation of the process using steered molecular dynamics produced a reliable result that can explain the molecular details of the breakdown process.

·2) Extraction of mRNA and membrane proteins from the living cell has been completed with success. This method is now being developed as a practical means to study the metabolism of a single living cell over an extended length of time, allowing periodical extraction of the molecules for biochemical analysis. Such procedure,especially that of extraction of membrane protein is not possible by any other method including one involving micromanipulators. Nano-mechanics of protein extraction is a very new accomplishment and the establishment of the upper and lower limits of the required force for extraction is an achievement of extraordinary importance. We also succeeded in extracting DNA from chromosomes under direct observation on a fluorescence microscope using the nano-manipulator constructed in this project. For further development of cellular level surgery, we are working on the development of a new method of boring a small hole on the cell membrane which can be used as a conduit for inserting mechanical probes into the cell and pulling them out of the cell.

9.Key Words
(1)atomic force microscope   (2)cell surgery   (3)cell manipulation
(4)mRNA harvest   (5)protein extension   (6)extraction of membrane protein
(7)protein nanomechanics   (8)chromosome nanomechanics   (9)single cell biology