Summary of Research Project Results under JSPS FY2001
"Research for the Future Program"

1.Research Institution Nagoya University
2.Research Area Life Sciences
3.Research Field Mechanisms of Plant Responses to Environment and Application to Biotechnology
4.Term of Project FY 1997 〜 FY 2001
5.Project Number 97L00601
6.Title of Project Organ Differentiation in Plants Responding to Environmental Stimuli

7.Project Leader
Name Institution,Department Title of Position
Yasunori Machida Nagoya University, Graduate School of Science Professor

8.Core Members

Names Institution,Department Title of Position
Kinya Toriyama Tohoku University, Graduate School of Agricultural Science Associate Professor
Sinobu Sato University of Tsukuba, Institute of Biological Sciences Associate Professor
Munetaka Sugiyama Tokyo University, Graduate School of Science Lecturer

9.Summary of Research Results

Dr. Machida shows that the 6b protein encoded by oncogene 6b is associatedwith tobacco transcription factor NtSIP1 and translocated into the tobacco nucleus. In addition, recent analysis have shown that the 6b protein also interacted with histon H3, which is supposed to be involved in expression of class1 knox homeobox genes such as KNAT2 and KNAT6. Dr. Tanaka shows that the rolB protein is specifically interacted with a certain member of the 14-3-3 family. Dr. Sugiyama shows that a mutation in the SRD2 gene causes defect of shoot formation from a hypocotyl of Arabidopsis by auxin and cytokinin. He has cloned a SRD2 gene. Dr.Morikami shows that root morphology is affected by high concentrations of sucrose and that the IBA gene is responsible for such alteration in morphology. Dr. Sato demonstrates that the AtC401 gene is responsible for stimulation of Arabidopsis flowering. Dr. Tasaka have cloned the CUC1 gene and demonstrates that it belongs to the NAC family. Dr. Ishiguro shows that the HLR gene is responsible for formation of the quiescent center of roots. Dr. Toriyama generated transgenic rice plants which have derivatives of the Ac transposable element in the rice genome and mapped these elements on the rice genome. Dr. Machida has developed negative marker genes for selection of mutants generated by a homology-based gene disruption. Currently, they have obtained a candidate clone for gene disruption.

10.Key Words

(1)Plants、(2)Dedifferentiation・regeneration、(3)Response to environment
(4)Agrobacterium oncogenes、(5)Phytohormones、(6)Light
(7)Sugar、(8)Cell proliferation、(9)Meristem