| 1.Research Institution | Nagoya University | |
| 2.Research Area | Life Sciences | |
| 3.Research Field | Mechanisms of Plant Responses to Environment and Application to Biotechnology | |
| 4.Term of Project | FY 1997 〜 FY 2001 | |
| 5.Project Number | 97L00601 | |
| 6.Title of Project | Organ Differentiation in Plants Responding to Environmental Stimuli |
| Name | Institution,Department | Title of Position |
| Yasunori Machida | Nagoya University, Graduate School of Science | Professor |
8.Core Members
| Names | Institution,Department | Title of Position |
| Kinya Toriyama | Tohoku University, Graduate School of Agricultural Science | Associate Professor |
| Sinobu Sato | University of Tsukuba, Institute of Biological Sciences | Associate Professor |
| Munetaka Sugiyama | Tokyo University, Graduate School of Science | Lecturer |
9.Summary of Research Results
|
Dr. Machida shows that the 6b protein encoded by oncogene 6b is associatedwith tobacco transcription
factor NtSIP1 and translocated into the tobacco nucleus. In addition, recent analysis have shown
that the 6b protein also interacted with histon H3, which is supposed to be involved in expression of
class1 knox homeobox genes such as KNAT2 and KNAT6. Dr. Tanaka shows that the rolB protein is
specifically interacted with a certain member of the 14-3-3 family. Dr. Sugiyama shows that a
mutation in the SRD2 gene causes defect of shoot formation from a hypocotyl of Arabidopsis by auxin
and cytokinin. He has cloned a SRD2 gene. Dr.Morikami shows that root morphology is affected by
high concentrations of sucrose and that the IBA gene is responsible for such alteration in
morphology. Dr. Sato demonstrates that the AtC401 gene is responsible for stimulation of
Arabidopsis flowering. Dr. Tasaka have cloned the CUC1 gene and demonstrates that it belongs to
the NAC family. Dr. Ishiguro shows that the HLR gene is responsible for formation of the quiescent
center of roots. Dr. Toriyama generated transgenic rice plants which have derivatives of the Ac
transposable element in the rice genome and mapped these elements on the rice genome. Dr.
Machida has developed negative marker genes for selection of mutants generated by a
homology-based gene disruption. Currently, they have obtained a candidate clone for gene disruption. |
10.Key Words
(1)Plants、(2)Dedifferentiation・regeneration、(3)Response to environment
(4)Agrobacterium oncogenes、(5)Phytohormones、(6)Light
(7)Sugar、(8)Cell proliferation、(9)Meristem