Summary of Research Project Results under JSPS FY2001
"Research for the Future Program"

1.Research Institution Osaka University
2.Research Area Integrated Fields
3.Research Field Biomedical Observation and Control
4.Term of Project FY 1997 〜 FY 2001
5.Project Number 97I00404
6.Title of Project Photonic Biomedical Sensing and Control

7.Project Leader
Name Institution,Department Title of Position
Satoshi Kawata Osaka University, Graduate school of Engineering Professor

8.Core Members

Names Institution,Department Title of Position
Osamu Nakamura Osaka University, Graduate school of Engineering Assistant Professor
Yasushi Inoue Osaka University, Graduate school of Engineering Research Assistant
Tadao Sugiura Nara Institute of Science and Technology, Graduate School of Information Science Assistant Professor

9.Summary of Research Results

1. A transcutaneous power transmission system has been developed which uses near-infrared light as a medium. This technique is for powering implanted devices such as cardiac pacemakers. The system was successfully applied to live rats. This technique was also applied to driving an optical transmitter for transcutaneous telemetry.
2. A real-time two-photon microscope using ultra-short laser pules has been developed. This microscope was used to image calcium ion concentration in living cells. With this microscope, in situ imaging of whole rat hearts was successfully performed, and abnormal calcium waves were discovered in injured hearts.
3. Near-infrared diffuse reflectance spectroscopy has been applied to different kinds of rat organs for their characterization. This technique was also used to differentiate diseased organs from normal ones.
4. A new ultrasound imaging technique has been proposed, which uses ultrasound caused by laser-induced breakdown of water. In order to cause the laser-induced breakdown, ultra-short laser pulses are focused in water. With this technique, real-time, high-resolution ultrasound imaging is possible.
5. Optical tomography using interactions between light and pulsed ultrasound has been developed. This technique was applied to a thick scattering sample. The spatial resolution of the measurement was less than 1 mm.
6. Ultra-short laser pulses have been used to induce calcium ion waves of in living HeLa cells. Calcium waves were imaged by fluorescence and were observed to propagate from the laser focal point inside the cell.
7. A microscope has been developed which images coherent anti-stokes Raman scattering by use of ultra-short laser pulses. This microscope can be used to observe molecular distribution in living cells without staining them.
8. A microscope has been developed which images second harmonic waves generated by ultra-short laser pulses. This microscope can image membrane potentials in living cells in real time.

10.Key Words

(1)near-infrared light、(2)biomedical measurements、(3)optical communication
(4)microscopy、(5)ultra-short laser pulses、(6)nonlinear optics
(7)calcium ion、(8)near-infrared spectroscopy、(9)ultrasound