Summary of Research Project Results under JSPS FY2001
"Research for the Future Program"



1.Research Institution Osaka University
 
2.Research Area Integrated Fields
 
3.Research Field Tissue Engineering
 
4.Term of Project FY 1998 〜 FY 2001
 
5.Project Number 98I00201
 
6.Title of Project Biological Tissue Engineering

7.Project Leader
Name Institution,Department Title of Position
Yukichi Umakoshi Osaka University, Graduate School of Engineering Professor

8.Core Members

Names Institution,Department Title of Position
Junzo Takahashi Osaka University, Graduate School of Dentistry Professor
Ken Ikeuchi Kyoto University, Institute for Frontier Medical Science Professor
Toshikazu Takigawa Kyoto University, Graduate School of Engineering Associate Professor

9.Summary of Research Results

The purpose of this project is to evaluate the regenerated tissue and organ and develop the evaluation method. Main results obtained by this project are as folows.
(1)Mechanical properties of dog's tracheas, artificial tracheas and regenerated tracheas were measured. Mechanical properties of artificial tracheas, regenerated tracheas were compared changing materials and shapes. Most favorable shape and materials of artificial tracheas were designed for development of regenerated tracheas.
(2)Preferential orientations of hydroxyapatite (HAp)crystallites in various biological normal and regenerated hard tissues were analyzed by the micro-beam X-ray diffractometer. HAp texture diffres in the normal hard tissue with different shapes under various stress conditions in vivo. Predominant alignment of c-axis in HAp was observed as one orientation along the longitudinal axis in a rabbit ulna and as two-dimensional orientation in a rabbit skull along the surface. Using this method the difference between natural and regenerated hard tissues can be evaluated.
(3)Using ultrasonic wave, Young's modulus, shape and thickness of cartilage can be measured. This new method can be applied for measurement of shape and damage of cartilage and evaluation of regenerated cartilage.
(4)To clarify the multiple functionality of connective tissue growth factor, the effects of nascent CTGF within the cell by transient expression were examined. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. This CTGF was operative for regeneration of various tissues and organ and it was very attractive for stimulating the regeneration process of bone, cartilage and blood vessel.

10.Key Words

(1)Bone、(2)Regeneration、(3)Cartilage
(4)Biological Tissue Engineering、(5)Growth factor、(6)Trachea
(7)Evaluation、(8)Mechanical Property、(9)Tissue


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