Summary of Research Project Results under JSPS FY2001 "Research for the Future Program"

Names	Institution,Department	Title of Position
Yutaka Seino	Kyoto university, Graduate School of Medicine, Department of Metabolism	Professor
Shunnosuke Hannda	Tokai UniversitySchool of Medicine, Department of Cardiology	Professor
Tsutomu Chiba	Kyoto University, Graduate school of Medicine, Department of Gastroenterology	Professor

　The purpose of this project was to develop of next stem cell-therapies to repair the organ functions in intractable diseases such as hematological malignancies, diabetes mellitus, ischemic heart disease and chronic intestinal inflammation using stem cell system. The stem cell system may provide high-functional, less invasive and lower expensive therapies for aged patients
We develop a novel co-culture culture system in which CD34+ cells were separated by filter membrane from mouse stromal cells as feeder cells. The fold of expansion was estimated by the NOD/SCID mouse repopulating assay. We planned a clinical research to evaluate the safety and efficacy of expanded CD34+ cells. We also developed a culture system for produce CD34+ cells from CD34 negative stem cells.
We established a fat cell line transduced with the insulin gene which responded to the concentration of surrounding sugar level. The cells enclosed in a capsule consisting of immuno-dissecting menbrane were implanted in intraperitoneal cavity of diabetic model mice. The diabetic state of the implanted mice was well controlled for months.
We isolated a cardiomyocyte cell line (CMG) from adult mouse bone marrow using 5-azathytidine which showed self-beating automatically. When the cardiomyocytes were transplanted into ischemic heart tissue, they survived and functioned in the cardiac tissue.
As to the regeneration of intestine, we develop a new drug-delivery system using microsphare to distribute cytokines specifically to small intestine. Using this DDS, we tried to regenerate the MALT tissues of intestine

1.Research Institution		Tokai University

2.Research Area		Integrated Fields

3.Research Field		Tissue Engineering

4.Term of Project		FY 1997 ～ FY 2001

5.Project Number		97I00201

6.Title of Project		Functional Regeneration by Medical Bioengineering

Name	Institution,Department	Title of Position
Tomomitsu Hotta	Tokai University School of Medicine, Department of Medicine	Professor

Summary of Research Project Results under JSPS FY2001"Research for the Future Program"

Summary of Research Project Results under JSPS FY2001
"Research for the Future Program"