Summary of Research Project Results Under the JSPS FY2000
"Research for the future Program"



1.Research Institution Nara Institute of Science and Technology
 
2.University-Industry Cooperative Research Committee 160th Committee on Plant Biotechnology on Environmental Changes to the Earth
 
3.Term of Project FY1996〜FY2000
 
4.Project Number 96R16001
 
5.Title of Project Molecular Breeding of Plants for Food, Resources and the Environment in the 21st Century

6.Projetct Leader
Name Institution,Department Title of Position
Isogai, Akira Nara Institute of Science and Technology, Graduate School of Biological Sciences Professor

7.Core Members

8.Cooperating Researchers

Names Institution,Department Title of Position
Shinmyo, Atsuhiko Nara Institute of Science and Technology, Graduate School of Biological Sciences Professor
Shimamoto, Ko Nara Institute of Science and Technology, Graduate School of Biological Sciences Professor
Kohchi, Takayuki Nara Institute of Science and Technology, Graduate School of Biological Sciences Associate Professor

9.Summary of Research Results

1. Purpose of this project
This project aims the developments and potential applications in the field of plant molecular breeding for food, resources and the environment in the 21st century.
2. Results of the project
(1) Screening of genetic resources:
Among many species of barley and wild plants, we screened plants with useful genetic properties such as salt tolerance and draught tolerance by field tests. Then we analyzed the genetics of plants. We made a data base of plants in the rain forest in terms of chemical constituents, flowering and other many useful properties.
(2) Molecular breeding of stress-tolerant plants:
We analyzed molecular basis of some stress tolerant plants and isolated many genes which are involved in the phenomenon. Furthermore, we analyzed the resistant mechanisms to pathogens and wounding, and found many genes in pathogens and plants. Then we transformed the genes to a plant and confirmed that the transformed plant acquired the tolerance of the biotic and abiotic stresses.
(3) Plants for phytoremediation:
Among of many water plants, we screened plants for phytoremediation and then tried to establish the process of transformation and regeneration of the plants.
(4) Production of useful plant constituents:
We isolated genes involved in biosynthesis of plant alkaloids and transformed the genes in plants. The transformed plant acquired an ability to produce the alkaloid with high effectiveness.

10.Key Words

(1)Molecular Breeding、(2)Gene Resources、(3)Stress Tolerance
(4)Disease Resistance、(5)Salt Tolerance、(6)Drought Tolerance
(7)Phytoremediation、(8)Alkaloid

11.References

[Reference Articles]
Author Title of Article
Fujita, M. Nutrient removal and starch production through cultivation of Wolffia arrhiza
Journal Volume Year Pages Concerned
J. Biosci. Bioeng. 87・2 1999 194-198

Author Title of Article
Mori, K. Plant regeneration from septum segment of a water plant Pak-bung (Ipomoea aquatica)
Journal Volume Year Pages Concerned
Jap. J. Water Tretament Biol. 35・1 1999 1-7

Author Title of Article
Shiraishi, T. Simple Assay for Formate Dehydrogenase Activity by Gas Chromatography-Mass Spectrometry (GC-MS)
Journal Volume Year Pages Concerned
Journal of Chromatography A 855 1999 337-340

Author Title of Article
Shiraishi, T. Formate Dehydrogenase in rice plant (Growth stimulation effect of formate on rice plant)
Journal Volume Year Pages Concerned
Journal of Bioscience and Bioengineering 89 2000 241-246

Author Title of Article
Shiraishi, T. Formate protects photosynthetic machinery from its photoinhibition.
Journal Volume Year Pages Concerned
Journal of Bioscience and Bioengineering 89 2000 564-568

Author Title of Article
Shiraishi, T. Formate dehydrogenase in Arabidopsis thaliana
Journal Volume Year Pages Concerned
Journal of Bioscience and Bioengineering 90 2000 691-693

Author Title of Article
Ogawa, M. Gibberellin responsive genes: High level of transcript accumulation in leaf sheath meristematic tissue from Zea mays L..
Journal Volume Year Pages Concerned
Plant Mol. Biol. 40 1999 654-657

Author Title of Article
Hatanaka, T. Transgenic plants of coffee Coffea canephora from embryogenic callus via Agrobacterium tumefaciens-mediated transformation.
Journal Volume Year Pages Concerned
Plant Cell Reports 19 1999 106-110

Author Title of Article
Hatanaka, T. Molecular cloning and characterization of coffee cDNA encoding spermidine synthase.
Journal Volume Year Pages Concerned
Plant Science 140 1999 161-168

Author Title of Article
Okushima, Y. Glycosylation and its adequate processing is critical for protein secretion in tobacco BY2 cells.
Journal Volume Year Pages Concerned
J. Plant Physiol. 154 1999 623-627

Author Title of Article
Okushima, Y. Secreted proteins of tobacco cultured BY2 cells: identification of a new member of pathogenesis-related proteins.
Journal Volume Year Pages Concerned
Plant Mol. Biol. 42 2000 479-488

Author Title of Article
Hara, K. Rapid systemic accumulation of transcripts encoding a tobacco WRKY transcription factor upon wounding.
Journal Volume Year Pages Concerned
Mol. Gen. Genet. 263 2000 30-37

Author Title of Article
Ogawa, M. Rice gibberillin-insensitive gene homolog, OsGAI, encodes a nuclear-localized protein capable of gene activation at transcriptional level
Journal Volume Year Pages Concerned
Gene 245 2000 21-29

Author Title of Article
Hara, K. Screening of wound-responsive genes identifies an immediate-early expressed gene encoding a highly charged protein in mechanically wounded tobacco plants.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 684-691

Author Title of Article
Ogata, S. Mutational analysis of the signal for a nuclear localization of proteins which accumulate specifically during meiosis in lily microsporocytes.
Journal Volume Year Pages Concerned
Plant Cell Rep. 19 1999 101-105

Author Title of Article
Mousavi, A. A novel glycine-rich protein is associated with starch grain accumulation during anther development.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 40 1999 406-416

Author Title of Article
Takahashi, A. Lesion-mimic mutants of rice with alterations in early signaling events of defense.
Journal Volume Year Pages Concerned
Plant J. 17 1999 535-545

Author Title of Article
Shimamoto, K. Programmed cell death in plants.
Journal Volume Year Pages Concerned
Plant Biotechnology 16 1999 49-53

Author Title of Article
Kawasaki, T. The small GTP-binding protein Rac is a regulator of cell death in plants.
Journal Volume Year Pages Concerned
Proc. Natl. Acad. Sci. USA 96 1999 10922-10926

Author Title of Article
Ono, E. Essential role of the small GTPase Rac in disease resistance of rice.
Journal Volume Year Pages Concerned
Proc. Natl. Acad. Sci. USA 98 2001 759-764

Author Title of Article
Fukushima, E. *Improved salt tolerance of transgenic tobacco expressing apoplastic yeast-derived invertase.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 42・2 2001 245-249

Author Title of Article
Ifuku, K. *N-terminal sequence of the extrinsic 23 kDa protein in photosynstem II has importance in ion-retention in oxygen-evolution.
Journal Volume Year Pages Concerned
Biochim. Biophys. Acta in press 2001  

Author Title of Article
Ifuku, K. *Isolation of cucumber cDNA (accession No. AB03225) for the 23kDa polypeptide of the oxygen-evolving complex of photosystem II (psbP) (PGR00-031).
Journal Volume Year Pages Concerned
Plant Physiol. 122 2000 619

Author Title of Article
Miyake, C. Determination of the rate of photoreduction of O2 in the water-water cycle in watermelon leaves and enhancement of the rate by limitation of photosynthesis.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 335-343

Author Title of Article
Kawasaki,, A. Responses of wild watermelon to drought stress: Accumulation of an argE homologue and citrulline in leaves in water deficits.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 864-873

Author Title of Article
Nakayama, H. Ectoine, the compatible solute of Halomonas elongata, confers hyperosmotic tolerance in cultured tobacco cells.
Journal Volume Year Pages Concerned
Plant Physiol. 122 2000 1239-1247

Author Title of Article
Horie, T. Two types of HKT homologues with different properties of Na+ and K+ transportation in Oryza sativa.
Journal Volume Year Pages Concerned
Plant J.     (in press)

Author Title of Article
Che, F. S. Biochemical and morphological features of rice cell death Induced by Pseudomonas avenae.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 40 1999 1036-1045

Author Title of Article
Che, F. S. Flagellin from an incompatible strain of Pseudomonas avenae induces a resistance response in cultured rice cells.
Journal Volume Year Pages Concerned
J. Biol. Chem. 275 2000 32347-32356

Author Title of Article
Suzuki, K. An Atropa belladonna hyoscyamine 6b-hydroxylase gene is differentially expressed in the root pericycle and anthers
Journal Volume Year Pages Concerned
Plant Mol. Biol. 40 1999 141-142

Author Title of Article
Shoji, T. Jasmonate induction of putrescine N-methyltransferase genes in the root of Nicotiana sylvestris
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 831-839

Author Title of Article
Shoji, T. Ethylene suppresses jasmonate-induced gene expression in nicotine biosynthesis
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 1072-1076

Author Title of Article
Sato, F. Metabolic engineering of plant alkaloid biosynthesis.
Journal Volume Year Pages Concerned
Proc. Natl. Acad. Sci. USA 98 2001 367-372

Author Title of Article
Kaneko, T. Worldwide distribution of beta-amylase thermostability in barley
Journal Volume Year Pages Concerned
Euphytica   2001 (in press)


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