Summary of Research Project Results Under the JSPS FY2000
"Research for the future Program"



1.Research Institution@Kyushu University
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2.Research Area@Life Science
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3.Research Field@Mechanisms of Plant Responses to Environment and Application to Biotechnology
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4.Term of Project@FY1996`FY2000
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5.Project Number@96L00602
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6.Title of Project@Genetically engineered enhancement of tolerance for environmental stresses in higher plants

7.Projetct Leader
Name Institution,Department Title of Position
Koh Iba Faculty of Science, Kyushu University Professor

8.Core Members

Names Institution,Department Title of Position
Takiko Shimada Research Institute of Agricultural Resources, Ishikawa Agricultural College Professor
Tetsuko Takabe Graduate School of Bioagricultural Sciences, Nagoya University Professor
Yuichi Takeuchi Graduate School of Science and Engineering, Hokkaido Tokai University Professor

9.Cooperating Researchers

Names Institution,Department Title of Position
Daisuke Takezawa Institute of Low Temperature Science, Hokkaido University Res. Asso.
Tomonabu Kusano Research and Education Center for Genetic Information, Nara Institute of Science and Technology Asso. Professor
Ikuo Nishida Department of Biological Sciences, Graduate School of Science, The University of Tokyo Asso. Professor

10.Summary of Research Results

Climate changes on a global scale have been predicted for the 21st century as the mean air temperature rises as a result of increasing concentrations of carbon dioxide and other trace greenhouse-effect gases in the atmosphere. Since agriculture and forestry are industries that are engaged in production through the utilization of the natural environment, productivity is highly susceptible to climate changes. Consequently, there are concerns over the effects of climate changes brought about by greenhouse effects. Although considerable research has been conducted to evaluate the effects of global warming on agriculture, further efforts are thus being made to search for specific and practical approaches to enhancing plants tolerance to high-temperature environments. In this project, novel genetic engineering approaches for imparting temperature, drought, UV tolerances to plants were developed. The techniques introduced here generates an efficacy that is not transient, but rather persists during plant growth. This efficacy is achieved through the direct control of intrinsic gene expression without introducing bacterial or other extrinsic genes. From these advantages, the techniques described here have the potential to evolve into a practical technology.

11.Key Words

(1)Higher PlantsA(2)Environmental StressesA(3)Genetic Engineering
(4)Climate ChangesA(5)Molecular BreedingA(6)Carbon Dioxide
(7)TemperatureA(8)DroughtA(9)UV

12.References

[Reference Articles]
Author Title of Article
T. Hamada Modification of fatty acid composition by over- and antisemse-expression of a microsomal -3 fatty acid desaturase gene in transgenic tobacco
Journal Volume Year Pages Concerned
Trans. Res. 5 1996 115-121

Author Title of Article
G. Horiguchi Expression of a gene for plastid -3 fatty acid desaturase and changes in lipid and fatty acid compositions in light- and dark-growr wheat leaves
Journal Volume Year Pages Concerned
Physiol. Plant. 96 1996 275-283

Author Title of Article
G. Horiguchi Role of -3 fatty acid desaturases in the regulation of the level of trienoic fatty acids during leaf cell maturation
Journal Volume Year Pages Concerned
Planta 199 1996 439-442

Author Title of Article
H. Inada Specific expression of the chloroplast gene for RNA polymerase (rpoB) at an early stage of leaf development in rice
Journal Volume Year Pages Concerned
Plant Cell Physiol. 37 1996 229-232

Author Title of Article
T. Hamada cDNA cloning of a wounding-inducible gene encoding a plastid -3 fatty acid dessaturase frome tobacco
Journal Volume Year Pages Concerned
Plant Cell Physiol. 37 1996 606-611

Author Title of Article
H. Kodama Structure, chromosomal location and expression of a rice gene encoding the microsome -3 fatty acid desaturase
Journal Volume Year Pages Concerned
Plant Mol. Biol. 33 1997 493-502

Author Title of Article
H. Inada Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid-encoded RNA polymerase gene (rpoB)
Journal Volume Year Pages Concerned
Plant J. 11 1997 883-890

Author Title of Article
T. Nishiuchi Wounding changes the spatial expression pattern of the arabidopsis plastid -3 fatty acid desaturase gene (FAD7) through different signal transduction pathways
Journal Volume Year Pages Concerned
Plant Cell 9 1997 1701-1712

Author Title of Article
K. Kusumi A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice
Journal Volume Year Pages Concerned
Plant J. 12 1997 1241-1250

Author Title of Article
T. Berberich Two maize genes encoding -3 fatty acid desaturase and their differential expression to temperature
Journal Volume Year Pages Concerned
Plant Mol. Biol. 36 1998 297-306

Author Title of Article
J. Kusumi Characterization of a nonsense mutation in FAD7, the gene which encodes -3 desaturase in Arabidopsis thaliana
Journal Volume Year Pages Concerned
J. Plant Res. 111 1998 87-91

Author Title of Article
G. Horiguchi Developmental regulation of genes for microsome and plastid -3 fatty acid desaturase in wheat (Triticum aestivum L.)
Journal Volume Year Pages Concerned
Plant Cell Physiol. 39 1998 540-544

Author Title of Article
Y. Wakita Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment
Journal Volume Year Pages Concerned
Genes genet. Syst. 73 1998 219-226

Author Title of Article
T. Fuse A Ti-plasmid-convertible phage vector system harboring the hygromycin B phosphotransferase gene
Journal Volume Year Pages Concerned
Plant Biotech. 15 1998 227-230

Author Title of Article
T. Hamada Characterization of transgenic tobacco with an increased -linolenic acid level
Journal Volume Year Pages Concerned
Plant Physiol. 118 1998 591-598

Author Title of Article
T. Nishiuchi Roles of plastid -3 fatty acid desaturases in defence response of higher plants
Journal Volume Year Pages Concerned
J. Plant Res. 111 1998 481-486

Author Title of Article
T. Ohtsubo Molecular cloning of AtMMH, an Arabidopsis thaliana ortholog of the Escherichia coli mutM gene, and analysis of functional domains of its product
Journal Volume Year Pages Concerned
Mol. Gen. Genet. 259 1998 577-590

Author Title of Article
T. Nishiuchi Wound-induced expression of the FAD7 gene is mediated by different regulatory domains of its promoter in leaves/ stems and roots
Journal Volume Year Pages Concerned
Plant Physiol. 121 1999 1239-1246

Author Title of Article
T. Shimada Modification of fatty acid composition in rice plants by transformation with a tobacco microsomal -3 fatty acid desaturase gene (NtFAD3)
Journal Volume Year Pages Concerned
Plant Biotech. 17 2000 43-48

Author Title of Article
H. Kodama Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid -3 fatty acid desaturase gene
Journal Volume Year Pages Concerned
Plant Sci. 155 2000 153-160

Author Title of Article
N. Araki Temperature-sensitive Arabidopsis mutant defective in 1-deoxy-D-xylulose 5-phosphate synthase within the plastid non-mevalonate pathway of isoprenoid biosynthesis
Journal Volume Year Pages Concerned
Physiol. Plant. 108 2000 19-24

Author Title of Article
K. Kusumi Characterization of a zebra mutant of rice having increased susceptibility of light stress
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 158-164

Author Title of Article
Y. Murakami Trienoic fatty acids and plant tolerance of high temperature
Journal Volume Year Pages Concerned
Science 287 2000 476-479

Author Title of Article
G. Horiguchi Temperature-dependent translational regulation of the ER -3 fatty acid desaturase gene in wheat root tips
Journal Volume Year Pages Concerned
Plant J. 24 2000 805-813

Author Title of Article
O. matsuda Hormonal regulation of tissue-specific ectopic expression of an Arabidopsis endoplasmic reticulum-type -3 fatty acid desaturase (FAD3) gene
Journal Volume Year Pages Concerned
Planta @ 2001 in press

Author Title of Article
M. Otani High frequency plant regeneration from leaf calli in sweet potato cv. Chugoku 25
Journal Volume Year Pages Concerned
Plant Tissue Culture Letters 13 1996 23-27

Author Title of Article
M. Otani Efficient embryogenic callus formation in sweet potato (Ipomoea batats (L. ) Lam. )
Journal Volume Year Pages Concerned
Breeding Science 46 1996 257-260

Author Title of Article
M. Otani Fertile transgenic plants of Ipomoea trichocarpa Ell. induced by different strains of Agrobacterium rhizogenes
Journal Volume Year Pages Concerned
Plant Science 116 1996 169-175

Author Title of Article
S. Takumi Production of transgenic wheat through particle bombardment of scutellar tissues: frequency is influenced by culture duration
Journal Volume Year Pages Concerned
J. Plant Physiol. 149 1996 418-423

Author Title of Article
T. Tsuchiya Transient expression of a reporter gene in bulbscales and immature embryos of three Lilium species is affected by 5' upstream sequences and culture conditions
Journal Volume Year Pages Concerned
Physiol. Plant. 98 1996 699-704

Author Title of Article
M. Yamagishi Distorted segregation of RFLP markers in regenerated plants derivec from anther culture of an F1 hybrid of rice
Journal Volume Year Pages Concerned
Genes and Genetic Systems 71 1996 37-41

Author Title of Article
M. Yamagishi A comparison of somaclonal variation in rice plants derived and not derived from protoplasts
Journal Volume Year Pages Concerned
Plant Breed. 115 1996 289-294

Author Title of Article
M. Yamagishi Gametoclonal variation in anther culture-derived rice plants: Mutatic breeding of new elite lines showing shortculm and early heading
Journal Volume Year Pages Concerned
J. Genet. Breed. 50 1996 269-275

Author Title of Article
T. Shimada Plant regeneration from leaf explants of Primula cuneifolia Hakusanensis, Hakusan-kozakura
Journal Volume Year Pages Concerned
Plant Biotech. 14 1997 47-50

Author Title of Article
S. Takumi Variation in transformation frequencies among six common wheat cultivars through particle bombardment of scutellar tissues
Journal Volume Year Pages Concerned
Genes and Genetic Systems 72 1997 63-69

Author Title of Article
S. Takumi The rice Actl promoter gave high activity of transient gusA expression in callus, immature embryos and pollen embryos of common wheat and its relatives following particle bombardment
Journal Volume Year Pages Concerned
Wheat Information Service 84 1997 25-32

Author Title of Article
T. Koba Isolation, identification and characterization of di somic and translocated barley chromosome addition lines of common wheat
Journal Volume Year Pages Concerned
Euphytica 96 1997 289-296

Author Title of Article
A. Makino Does decrease in ribulose-1, 5-biphosphate carboxylase by antisense RbcS lead to a higher N-use efficiency of photosynthesis under conditions of saturating CO2 and light in rice plants?
Journal Volume Year Pages Concerned
Plant Physiol. 114 1997 483-491

Author Title of Article
K. Murai Effects of barley chromosome on heading characters in wheat-barley chromosome addition lines
Journal Volume Year Pages Concerned
Euphytic 96 1997 281-287

Author Title of Article
N. Nagatani Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice
Journal Volume Year Pages Concerned
Plant Science 124 1997 49-56

Author Title of Article
N. Nagatani DNA derivery into rice cells and transformation using silicon carbide whiskers
Journal Volume Year Pages Concerned
Biotechnology Techniques 11 1997 471-473

Author Title of Article
M. Yamagishi Characteristics of genetic variation in the progenies of protoplast derived plants of rice, Oryza sativa cv. Nipponbare
Journal Volume Year Pages Concerned
Theor. Appl. Genet. 94 1997 1-7

Author Title of Article
M. Otani Transgenic plant production from embryogenic callus of sweet potato (Ipomoea batats (L. ) Lam. ) using Agrobacterium tumefaciens
Journal Volume Year Pages Concerned
Plant Biotech. 15 1998 11-16

Author Title of Article
M. Otani Embryogenic callus formation from immature embryo of Japanese morning glory (Pharbitis nil Choisy)
Journal Volume Year Pages Concerned
Plant Biotech. 15 1998 127-130

Author Title of Article
Y. Wakita Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment
Journal Volume Year Pages Concerned
Genes and Genetic Systems 73 1998 219-226

Author Title of Article
M. Yamagishi Chromosomal regions controlling anther culturability in rice (Oryza sativa L. )
Journal Volume Year Pages Concerned
Euphytica 103 1998 227-234

Author Title of Article
J. Matsushita Transgenic plant regeneration through silicon carbide whisker-mediated transformation of rice (Oryza sativa L. )
Journal Volume Year Pages Concerned
Breeding Science 49 1999 21-26

Author Title of Article
K. Tsunewaki Transfer of Triticum urartu cytoplasm to emmer wheat is difficult, if not impossible
Journal Volume Year Pages Concerned
Wheat Information Service 88 1998 27-31

Author Title of Article
T. Shimada Modification of fatty acid composition in rice transformation with a tobacco microsomal -3 fatty acid desaturase gene (NtFAD3)
Journal Volume Year Pages Concerned
Plant Biotech. 17 2000 43-48

Author Title of Article
A. Makino Whole-plant growth and N allocation in transgenic rice plants with decreased content of ribulose-1, 5-biphosphate carboxylase under different CO2 partial pressures
Journal Volume Year Pages Concerned
Aust. J. Plant Physiol. 27 2000 1-12

Author Title of Article
A, Makino Photosynthesis, plant growth and N allocation in transgenic rice plants with decreased Rubisco under CO2 enrichmen
Journal Volume Year Pages Concerned
J. Exp. Botany 51 2000 383-389

Author Title of Article
P. Sripichitt Improved response of anther culture in an indica rice cultivar of Thailand
Journal Volume Year Pages Concerned
Plant Prod. Sci. 33 2000 254-256

Author Title of Article
Y. Wakita A Tobacco microsomal -3 fatty acid desaturase gene increases the linolenic acid content in transgenic sweet potato (Ipomoea batatas)
Journal Volume Year Pages Concerned
Plant Cell Rep. @ 2001 in press.

Author Title of Article
T. Nakamura Distribution of glycinebetaine in old and young leaf blades of a salt-stressed barley plant
Journal Volume Year Pages Concerned
Plant Cell Physiol. 37 1996 873-877

Author Title of Article
P. Harinasut Exogenous glycinebetaine accumulation and increased salt tolerance in rice seedlings
Journal Volume Year Pages Concerned
Biosci. Biotec. Biochem. 60 1996 366-369

Author Title of Article
T. Nakamura Expression of betaine aldehyde dehydrogenase gene in rice, glycinebetaine nonaccumulator, and possible localization of its protein in peroxisomes
Journal Volume Year Pages Concerned
Plant J. 11 1997 1115-1120

Author Title of Article
M. Nomura Transgenically produced glycinebetaine protects ribulose 1, 5-bisphosphate carboxylase/oxygenase from inactivation in Synechococcus sp. PCC7942 under salt stress
Journal Volume Year Pages Concerned
Plant Cell Physiol. 39 1998 425-432

Author Title of Article
T. Hibino Molecular characterization of DnaK from the halotolerant cyanobacterium Aphanothece halophytica for ATPase, protein folding, and copper binding under various salinity conditions
Journal Volume Year Pages Concerned
Plant Mol. Biol. 40 1999 409-418

Author Title of Article
N. Kaku Expression of nhaAv gene encoding Na+/H+ antiporter from Vibrio alginolyticus in a freshwater cyanobacterium Synechococcus sp. PCC 7942 confers litium tolerance, but not sodium tolerance
Journal Volume Year Pages Concerned
Plant Cell Physiol. 40 1999 557-564

Author Title of Article
Y. Tanaka Salt tolerance of transgenic rice overexpressing yeast mitochondrial Mn-SOD in chloroplasts
Journal Volume Year Pages Concerned
Plant Science 148 1999 131-138

Author Title of Article
M. Sugino Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica acquires resistance to salt stress in transgenic tobacco plants
Journal Volume Year Pages Concerned
Plant Science 137 1999 81-88

Author Title of Article
Y. Muramoto Enhanced expression of a nuclease gene in leaves of barley plants under salt stress
Journal Volume Year Pages Concerned
Gene 234 1999 315-321

Author Title of Article
S. Kishitani Compatibility of glycinebetaine in rice plants:evaluation using transgenic rice plants with a gene for peroxisomal betaine aldehyde dehydrogenase from barley
Journal Volume Year Pages Concerned
Plant Cell Environ. 23 2000 107-114

Author Title of Article
H. Hoshida Enhanced tolerance to salt stress in transgenic rice that overexpressed chloroplast glutamine synthetase
Journal Volume Year Pages Concerned
Plant Mol. Biol. 43 2000 103-111

Author Title of Article
T. Hibino Molecular cloning and functional characterization of two kinds of betaine aldehyde dehydrogenase in betaine accumulating mangrove Avicennia marina(Forsk. )Vierh
Journal Volume Year Pages Concerned
Plant Mol. Biol. @ 2001 in press

Author Title of Article
K. Ono Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances the high-temperature tolerance o:tobacco during germination and early growth
Journal Volume Year Pages Concerned
Plant Science @ 2001 in press

Author Title of Article
Y. Takeuchi Induction and repair of damage to DNA in cucumber cotyledons irradiated with UV-B
Journal Volume Year Pages Concerned
Plant Cell Physiol. 37 1996 181-187

Author Title of Article
Y. Takeuchi The photorepair and photoisomerization of DNA lesions in etiolated cucumber cotyledons after irradiation by UV-B depends on wavelength
Journal Volume Year Pages Concerned
Plant Cell Physiol. 37 1998 745-750

Author Title of Article
M. Hada Action spectra of DNA photolyases for photorepair of cyclobutane pyrimidine dimers in sorghum and cucumber
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 644-648

Author Title of Article
Y. Takeuchi Formation of DNA lesions in cucumber cotyledons exposed to solar UV radiation
Journal Volume Year Pages Concerned
Environ. Sci. 13 2000 351-355

Author Title of Article
T. Fujibe Accumulation of pathogen-related proteins in tobacco leaves irradiated with UV-B
Journal Volume Year Pages Concerned
J. Plant Res. 113 2000 387-394

Author Title of Article
T. Berberich Cycloheximide induces a subset of low temperature inducible genes in maize
Journal Volume Year Pages Concerned
Mol. Gen. Genet. 254 1997 275-283

Author Title of Article
T. Berberich Two maize genes encoding plastidic -3 fatty acid desaturase and their differential expression to temperature
Journal Volume Year Pages Concerned
Plant Mol. Biol. 36 1998 297-306

Author Title of Article
T. Kusano Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein
Journal Volume Year Pages Concerned
Biochim. Biophys. Acta 1395 1998 171-175

Author Title of Article
K. Ito Cold-inducible bZIP protein gene in radish root regulated by calcium-and cycloheximide-mediated signals
Journal Volume Year Pages Concerned
Plant Science 142 1999 57-66

Author Title of Article
T. Berberich Involvement of a MAP kinase, ZmMPK5, in senescence and recovery from low-temperature-stress in maize
Journal Volume Year Pages Concerned
Mol. Gen. Genet. 262 1999 534-542

Author Title of Article
H. S. Yang The darkness-induced and senescence-associated gene, Ntdin, encodes a protein functioning in molybdenum cofactor biosynthesis in tabacco plants
Journal Volume Year Pages Concerned
@ @ 2001 submitted.

Author Title of Article
H. S. Yang Guard cell-and flower-specific expression of a tobacco gene, tbzF, encoding a bZIP-type transcriptional activator
Journal Volume Year Pages Concerned
@ @ 2001 submitted

Author Title of Article
I. Nishida A second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, Cucurbita moschata cv. Shirogikuza, codes for an oleate-selective isozyme: molecular cloning and protein purification studies
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 1381-1391

Author Title of Article
Y. Fujiki Multiple signaling pathways in gene expression during sugar starvation: pharmacological analysis of din gene expression in suspension-cultured cells of Arabidopsis
Journal Volume Year Pages Concerned
Plant Physiol. 124 2000 1139-1147

Author Title of Article
S. Yoshida Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana
Journal Volume Year Pages Concerned
Plant Cell Physiol. 42 2001 170-178

Author Title of Article
Y. Fujiki Dark-inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars.
Journal Volume Year Pages Concerned
Physiol. Plant. 111 2001 345-352

Author Title of Article
A. P. Tumbul Analysis of the structure, substrate specificity and mechanism of the glycerol-3-phosphate 1-acyltransferase from squash (Cucurbita moschata)
Journal Volume Year Pages Concerned
Acta Cryst. D57 2001 451-453

Author Title of Article
M. K. Watahiki The massugul mutation of Arabidopsis identified with failure of auxin-induced growth curvature of hypocotyl confers auxin insensitivity to hypocotyl and leaf
Journal Volume Year Pages Concerned
Plant Physiol. 115 1997 419-426

Author Title of Article
H. Hashimoto Three more members of the Aux/IAA gene family from mung bean (Vigna radiata) hypocotyl (Accession Nos. AB004931, AB004932 and AB004933) (PGR 97-137)
Journal Volume Year Pages Concerned
Plant Physiol. 115 1997 315

Author Title of Article
T. Kurata Light-stimulated root elongation in Arabidopsis thaliana
Journal Volume Year Pages Concerned
J. Plant Physiol. 151 1997 346-351

Author Title of Article
T. Kurata petitl, a conditional growth mutant of Arabidopsis defective in sucrose-dependent elongation growth
Journal Volume Year Pages Concerned
Plant Physiol. 118 1998 793-801

Author Title of Article
H. Hashimoto An auxin down-regulated mRNA from mung bean (Vigna radiata) hypocotyl (Accession No. AB012110) is related to an aluminum-inducible mRNA in wheat root (PGR 98-094)
Journal Volume Year Pages Concerned
Plant Physiol. 117 1998 717

Author Title of Article
M. Yamamoto Differential effects of 1-naphthaleneacetic acid, indole-3-acetic acid and 2, 4-dichlorophenoxyacetic acid on the gravitropic response of roots in an auxin-resistant mutant of Arabidopsis, aux1
Journal Volume Year Pages Concerned
Plant Cell Physiol. 39 1998 660-664

Author Title of Article
M. Yamamoto Effects of natural and synthetic auxins on the agravitropic growth habit of roots in auxin-resistant mutants of Arabidopsis, axr1 and axr4: Evidence for defects in the auxin influx mechanism of axr4
Journal Volume Year Pages Concerned
J. Plant Res. 112 1999 391-396

Author Title of Article
H. Hashimoto Isolation and expression of an elongation-dependent gene of mung bean (Vigna radiata) hypocotyl
Journal Volume Year Pages Concerned
Physiol. Plant. 106 1999 224-231

Author Title of Article
M. K. Watahiki, The MSG1 and AXR1 genes of Arabidopsis are likely to act independently in growth-curvature responses of hypocotyls
Journal Volume Year Pages Concerned
Planta 207 1999 362-369

Author Title of Article
R. M. Harper The NPH4 locus encodes the auxin response factor ARF7, a conditional regulator of differential growth in aerial Arabidopsis tissue
Journal Volume Year Pages Concerned
Plant Cell 12 2000 757-770

Author Title of Article
K. Fujihira An agravitropic mutant of Arabidopsis, endodermal amyloplast-lessl, that lacks amyloplasts in hypocotyl endodermal cell layer
Journal Volume Year Pages Concerned
Plant Cell Physiol. 41 2000 1193-1199

Author Title of Article
T. Fujibe Accumulation of pathogenesis-related proteins in tobacco leaves irradiated with UV-B
Journal Volume Year Pages Concerned
J. Plant Res. 113 2000 387-394

Author Title of Article
S. Yoshida Mechanism of adaptation to chilling temperatures in plants
Journal Volume Year Pages Concerned
Cryobiology and Cryotechnology 42 1996 45-48

Author Title of Article
M. Koike Accumulation of 19-kDa plasma membrane polypeptide during induction of freezing tolerance in wheat suspension-cultured cells by abscisic acid
Journal Volume Year Pages Concerned
Plant Cell Physiol. 38 1997 707-716

Author Title of Article
M. Murai Vacuolar membrane lesions induced by a freeze-thaw cycle in protoplasts isolated from deacclimated tubers of Jerusalem artichoke (Helianthus tuberosus L. )
Journal Volume Year Pages Concerned
Plant Cell Physiol. 39 1998 87-96

Author Title of Article
M. Murai Evidence for the cell wall involvement in temporal changes in freezing tolerance of Jerusalem artichoke (Helianthus tuberosus L. ) tubers during cold acclimation
Journal Volume Year Pages Concerned
Plant Cell Physiol. 39 1998 97-105

Author Title of Article
S. Yoshida A prefatory note on responses of plants to low temperature stress
Journal Volume Year Pages Concerned
J. Plant Res. 112 1999 223-224

Author Title of Article
C. Kuwabara Abscisic acid-induced secretory protieins in suspnesion-cultured cell of winter wheat
Journal Volume Year Pages Concerned
Plant Cell Physiol. 40 1999 184-191

Author Title of Article
D. Takezawa Elicitor and A23187-induced expression of WCK-1, a gene encoding mitogen-activated protein kinase in wheat
Journal Volume Year Pages Concerned
Plant Mol. Biol. 40 1999 921-933

Author Title of Article
S. Yoshida Alterations of intracellular pH in response to low temperature stresses
Journal Volume Year Pages Concerned
J. Plant Res. 112 1999 225-236

Author Title of Article
N. Ukaji Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation
Journal Volume Year Pages Concerned
Plant Physiol. 120 1999 481-490

Author Title of Article
M. Nagao Akinete formation in Tribonema bombycinum Derbes et Solier (Xanthophyceae) in relation to freezing tolerance
Journal Volume Year Pages Concerned
J. Plant Res. 112 1999 163-174

Author Title of Article
Y. Kawamura Tissue specificity of E subunit isoforms of plant vacuolar H+- ATPase and existence of isotype enzymes
Journal Volume Year Pages Concerned
J. Biol. Chem. 275 2000 6515-6522

Author Title of Article
D. Takezawa A rapid induction by elicitors of the mRNA encoding CCD-1, a 14 kDa Ca2+-binding protein in wheat cultured cells
Journal Volume Year Pages Concerned
Plant Mol. Biol. 42 2000 807-817

[Reference Books]
Author Title of Book
M. Otani Biotechnology in Agriculture and Forestry
Publisher Year Pages
Springer-Verlag 2000 in press

Author Title of Book
M. Otani Biotechnology in Agriculture and Forestry
Publisher Year Pages
Springer-Verlag 2000 in press

Author Title of Book
M. Otani Transgenic Plants
Publisher Year Pages
Maecel Dekker Inc. 2000 in press

Author Title of Book
K. Hotsubo Plant Cold Hardiness
Publisher Year Pages
Plenum Press 1998 8

Author Title of Book
N. Ukaji Methods in Electron Microscopy and Cytochemistry
Publisher Year Pages
Humana Press 2000 14

Author Title of Book
I. Nishida Physiology, Biochemistry and Molecular Biology of Plant Lipids
Publisher Year Pages
Kluwer Academic Publishers 1997 3

Author Title of Book
I. Nishida Advances in Plant Lipid Research
Publisher Year Pages
Universidad de Sevilla 1998 4


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