| 1.Research Institution | Kyushu University | |
| 2.Research Area | Life Science | |
| 3.Research Field | Mechanisms of Plant Responses to Environment and Application to Biotechnology | |
| 4.Term of Project | FY1996〜FY2000 | |
| 5.Project Number | 96L00602 | |
| 6.Title of Project | Genetically engineered enhancement of tolerance for environmental stresses in higher plants |
| Name | Institution,Department | Title of Position |
| Koh Iba | Faculty of Science, Kyushu University | Professor |
8.Core Members
| Names | Institution,Department | Title of Position |
| Takiko Shimada | Research Institute of Agricultural Resources, Ishikawa Agricultural College | Professor |
| Tetsuko Takabe | Graduate School of Bioagricultural Sciences, Nagoya University | Professor |
| Yuichi Takeuchi | Graduate School of Science and Engineering, Hokkaido Tokai University | Professor |
9.Cooperating Researchers
| Names | Institution,Department | Title of Position |
| Daisuke Takezawa | Institute of Low Temperature Science, Hokkaido University | Res. Asso. |
| Tomonabu Kusano | Research and Education Center for Genetic Information, Nara Institute of Science and Technology | Asso. Professor |
| Ikuo Nishida | Department of Biological Sciences, Graduate School of Science, The University of Tokyo | Asso. Professor |
10.Summary of Research Results
|
Climate changes on a global scale have been predicted for the 21st century as the mean air temperature rises as a result of increasing concentrations of carbon dioxide and other trace greenhouse-effect gases in the atmosphere. Since agriculture and forestry are industries that are engaged in production through the utilization of the natural environment, productivity is highly susceptible to climate changes. Consequently, there are concerns over the effects of climate changes brought about by greenhouse effects. Although considerable research has been conducted to evaluate the effects of global warming on agriculture, further efforts are thus being made to search for specific and practical approaches to enhancing plants ユ tolerance to high-temperature environments. In this project, novel genetic engineering approaches for imparting temperature, drought, UV tolerances to plants were developed. The techniques introduced here generates an efficacy that is not transient, but rather persists during plant growth. This efficacy is achieved through the direct control of intrinsic gene expression without introducing bacterial or other extrinsic genes. From these advantages, the techniques described here have the potential to evolve into a practical technology. |
11.Key Words
(1)Higher Plants、(2)Environmental Stresses、(3)Genetic Engineering
(4)Climate Changes、(5)Molecular Breeding、(6)Carbon Dioxide
(7)Temperature、(8)Drought、(9)UV
12.References
| Author | Title of Article | |||
| T. Hamada | Modification of fatty acid composition by over- and antisemse-expression of a microsomal ω-3 fatty acid desaturase gene in transgenic tobacco | |||
| Journal | Volume | Year | Pages Concerned | |
| Trans. Res. | 5 | 1996 | 115-121 | |
| Author | Title of Article | |||
| G. Horiguchi | Expression of a gene for plastid ω-3 fatty acid desaturase and changes in lipid and fatty acid compositions in light- and dark-growr wheat leaves | |||
| Journal | Volume | Year | Pages Concerned | |
| Physiol. Plant. | 96 | 1996 | 275-283 | |
| Author | Title of Article | |||
| G. Horiguchi | Role of ω-3 fatty acid desaturases in the regulation of the level of trienoic fatty acids during leaf cell maturation | |||
| Journal | Volume | Year | Pages Concerned | |
| Planta | 199 | 1996 | 439-442 | |
| Author | Title of Article | |||
| H. Inada | Specific expression of the chloroplast gene for RNA polymerase (rpoB) at an early stage of leaf development in rice | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 37 | 1996 | 229-232 | |
| Author | Title of Article | |||
| T. Hamada | cDNA cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid dessaturase frome tobacco | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 37 | 1996 | 606-611 | |
| Author | Title of Article | |||
| H. Kodama | Structure, chromosomal location and expression of a rice gene encoding the microsome ω-3 fatty acid desaturase | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 33 | 1997 | 493-502 | |
| Author | Title of Article | |||
| H. Inada | Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid-encoded RNA polymerase gene (rpoB) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant J. | 11 | 1997 | 883-890 | |
| Author | Title of Article | |||
| T. Nishiuchi | Wounding changes the spatial expression pattern of the arabidopsis plastid ω-3 fatty acid desaturase gene (FAD7) through different signal transduction pathways | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell | 9 | 1997 | 1701-1712 | |
| Author | Title of Article | |||
| K. Kusumi | A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant J. | 12 | 1997 | 1241-1250 | |
| Author | Title of Article | |||
| T. Berberich | Two maize genes encoding ω-3 fatty acid desaturase and their differential expression to temperature | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 36 | 1998 | 297-306 | |
| Author | Title of Article | |||
| J. Kusumi | Characterization of a nonsense mutation in FAD7, the gene which encodes ω-3 desaturase in Arabidopsis thaliana | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 111 | 1998 | 87-91 | |
| Author | Title of Article | |||
| G. Horiguchi | Developmental regulation of genes for microsome and plastid ω-3 fatty acid desaturase in wheat (Triticum aestivum L.) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 39 | 1998 | 540-544 | |
| Author | Title of Article | |||
| Y. Wakita | Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment | |||
| Journal | Volume | Year | Pages Concerned | |
| Genes genet. Syst. | 73 | 1998 | 219-226 | |
| Author | Title of Article | |||
| T. Fuse | A Ti-plasmid-convertible λ phage vector system harboring the hygromycin B phosphotransferase gene | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Biotech. | 15 | 1998 | 227-230 | |
| Author | Title of Article | |||
| T. Hamada | Characterization of transgenic tobacco with an increased α-linolenic acid level | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 118 | 1998 | 591-598 | |
| Author | Title of Article | |||
| T. Nishiuchi | Roles of plastid ω-3 fatty acid desaturases in defence response of higher plants | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 111 | 1998 | 481-486 | |
| Author | Title of Article | |||
| T. Ohtsubo | Molecular cloning of AtMMH, an Arabidopsis thaliana ortholog of the Escherichia coli mutM gene, and analysis of functional domains of its product | |||
| Journal | Volume | Year | Pages Concerned | |
| Mol. Gen. Genet. | 259 | 1998 | 577-590 | |
| Author | Title of Article | |||
| T. Nishiuchi | Wound-induced expression of the FAD7 gene is mediated by different regulatory domains of its promoter in leaves/ stems and roots | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 121 | 1999 | 1239-1246 | |
| Author | Title of Article | |||
| T. Shimada | Modification of fatty acid composition in rice plants by transformation with a tobacco microsomal ω-3 fatty acid desaturase gene (NtFAD3) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Biotech. | 17 | 2000 | 43-48 | |
| Author | Title of Article | |||
| H. Kodama | Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Sci. | 155 | 2000 | 153-160 | |
| Author | Title of Article | |||
| N. Araki | Temperature-sensitive Arabidopsis mutant defective in 1-deoxy-D-xylulose 5-phosphate synthase within the plastid non-mevalonate pathway of isoprenoid biosynthesis | |||
| Journal | Volume | Year | Pages Concerned | |
| Physiol. Plant. | 108 | 2000 | 19-24 | |
| Author | Title of Article | |||
| K. Kusumi | Characterization of a zebra mutant of rice having increased susceptibility of light stress | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 41 | 2000 | 158-164 | |
| Author | Title of Article | |||
| Y. Murakami | Trienoic fatty acids and plant tolerance of high temperature | |||
| Journal | Volume | Year | Pages Concerned | |
| Science | 287 | 2000 | 476-479 | |
| Author | Title of Article | |||
| G. Horiguchi | Temperature-dependent translational regulation of the ER ω-3 fatty acid desaturase gene in wheat root tips | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant J. | 24 | 2000 | 805-813 | |
| Author | Title of Article | |||
| O. matsuda | Hormonal regulation of tissue-specific ectopic expression of an Arabidopsis endoplasmic reticulum-type ω-3 fatty acid desaturase (FAD3) gene | |||
| Journal | Volume | Year | Pages Concerned | |
| Planta | 2001 | in press | ||
| Author | Title of Article | |||
| M. Otani | High frequency plant regeneration from leaf calli in sweet potato cv. Chugoku 25 | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Tissue Culture Letters | 13 | 1996 | 23-27 | |
| Author | Title of Article | |||
| M. Otani | Efficient embryogenic callus formation in sweet potato (Ipomoea batats (L. ) Lam. ) | |||
| Journal | Volume | Year | Pages Concerned | |
| Breeding Science | 46 | 1996 | 257-260 | |
| Author | Title of Article | |||
| M. Otani | Fertile transgenic plants of Ipomoea trichocarpa Ell. induced by different strains of Agrobacterium rhizogenes | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Science | 116 | 1996 | 169-175 | |
| Author | Title of Article | |||
| S. Takumi | Production of transgenic wheat through particle bombardment of scutellar tissues: frequency is influenced by culture duration | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Physiol. | 149 | 1996 | 418-423 | |
| Author | Title of Article | |||
| T. Tsuchiya | Transient expression of a reporter gene in bulbscales and immature embryos of three Lilium species is affected by 5' upstream sequences and culture conditions | |||
| Journal | Volume | Year | Pages Concerned | |
| Physiol. Plant. | 98 | 1996 | 699-704 | |
| Author | Title of Article | |||
| M. Yamagishi | Distorted segregation of RFLP markers in regenerated plants derivec from anther culture of an F1 hybrid of rice | |||
| Journal | Volume | Year | Pages Concerned | |
| Genes and Genetic Systems | 71 | 1996 | 37-41 | |
| Author | Title of Article | |||
| M. Yamagishi | A comparison of somaclonal variation in rice plants derived and not derived from protoplasts | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Breed. | 115 | 1996 | 289-294 | |
| Author | Title of Article | |||
| M. Yamagishi | Gametoclonal variation in anther culture-derived rice plants: Mutatic breeding of new elite lines showing shortculm and early heading | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Genet. Breed. | 50 | 1996 | 269-275 | |
| Author | Title of Article | |||
| T. Shimada | Plant regeneration from leaf explants of Primula cuneifolia Hakusanensis, Hakusan-kozakura | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Biotech. | 14 | 1997 | 47-50 | |
| Author | Title of Article | |||
| S. Takumi | Variation in transformation frequencies among six common wheat cultivars through particle bombardment of scutellar tissues | |||
| Journal | Volume | Year | Pages Concerned | |
| Genes and Genetic Systems | 72 | 1997 | 63-69 | |
| Author | Title of Article | |||
| S. Takumi | The rice Actl promoter gave high activity of transient gusA expression in callus, immature embryos and pollen embryos of common wheat and its relatives following particle bombardment | |||
| Journal | Volume | Year | Pages Concerned | |
| Wheat Information Service | 84 | 1997 | 25-32 | |
| Author | Title of Article | |||
| T. Koba | Isolation, identification and characterization of di somic and translocated barley chromosome addition lines of common wheat | |||
| Journal | Volume | Year | Pages Concerned | |
| Euphytica | 96 | 1997 | 289-296 | |
| Author | Title of Article | |||
| A. Makino | Does decrease in ribulose-1, 5-biphosphate carboxylase by antisense RbcS lead to a higher N-use efficiency of photosynthesis under conditions of saturating CO2 and light in rice plants? | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 114 | 1997 | 483-491 | |
| Author | Title of Article | |||
| K. Murai | Effects of barley chromosome on heading characters in wheat-barley chromosome addition lines | |||
| Journal | Volume | Year | Pages Concerned | |
| Euphytic | 96 | 1997 | 281-287 | |
| Author | Title of Article | |||
| N. Nagatani | Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Science | 124 | 1997 | 49-56 | |
| Author | Title of Article | |||
| N. Nagatani | DNA derivery into rice cells and transformation using silicon carbide whiskers | |||
| Journal | Volume | Year | Pages Concerned | |
| Biotechnology Techniques | 11 | 1997 | 471-473 | |
| Author | Title of Article | |||
| M. Yamagishi | Characteristics of genetic variation in the progenies of protoplast derived plants of rice, Oryza sativa cv. Nipponbare | |||
| Journal | Volume | Year | Pages Concerned | |
| Theor. Appl. Genet. | 94 | 1997 | 1-7 | |
| Author | Title of Article | |||
| M. Otani | Transgenic plant production from embryogenic callus of sweet potato (Ipomoea batats (L. ) Lam. ) using Agrobacterium tumefaciens | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Biotech. | 15 | 1998 | 11-16 | |
| Author | Title of Article | |||
| M. Otani | Embryogenic callus formation from immature embryo of Japanese morning glory (Pharbitis nil Choisy) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Biotech. | 15 | 1998 | 127-130 | |
| Author | Title of Article | |||
| Y. Wakita | Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment | |||
| Journal | Volume | Year | Pages Concerned | |
| Genes and Genetic Systems | 73 | 1998 | 219-226 | |
| Author | Title of Article | |||
| M. Yamagishi | Chromosomal regions controlling anther culturability in rice (Oryza sativa L. ) | |||
| Journal | Volume | Year | Pages Concerned | |
| Euphytica | 103 | 1998 | 227-234 | |
| Author | Title of Article | |||
| J. Matsushita | Transgenic plant regeneration through silicon carbide whisker-mediated transformation of rice (Oryza sativa L. ) | |||
| Journal | Volume | Year | Pages Concerned | |
| Breeding Science | 49 | 1999 | 21-26 | |
| Author | Title of Article | |||
| K. Tsunewaki | Transfer of Triticum urartu cytoplasm to emmer wheat is difficult, if not impossible | |||
| Journal | Volume | Year | Pages Concerned | |
| Wheat Information Service | 88 | 1998 | 27-31 | |
| Author | Title of Article | |||
| T. Shimada | Modification of fatty acid composition in rice transformation with a tobacco microsomal ω-3 fatty acid desaturase gene (NtFAD3) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Biotech. | 17 | 2000 | 43-48 | |
| Author | Title of Article | |||
| A. Makino | Whole-plant growth and N allocation in transgenic rice plants with decreased content of ribulose-1, 5-biphosphate carboxylase under different CO2 partial pressures | |||
| Journal | Volume | Year | Pages Concerned | |
| Aust. J. Plant Physiol. | 27 | 2000 | 1-12 | |
| Author | Title of Article | |||
| A, Makino | Photosynthesis, plant growth and N allocation in transgenic rice plants with decreased Rubisco under CO2 enrichmen | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Exp. Botany | 51 | 2000 | 383-389 | |
| Author | Title of Article | |||
| P. Sripichitt | Improved response of anther culture in an indica rice cultivar of Thailand | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Prod. Sci. | 33 | 2000 | 254-256 | |
| Author | Title of Article | |||
| Y. Wakita | A Tobacco microsomal ω-3 fatty acid desaturase gene increases the linolenic acid content in transgenic sweet potato (Ipomoea batatas) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Rep. | 2001 | in press. | ||
| Author | Title of Article | |||
| T. Nakamura | Distribution of glycinebetaine in old and young leaf blades of a salt-stressed barley plant | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 37 | 1996 | 873-877 | |
| Author | Title of Article | |||
| P. Harinasut | Exogenous glycinebetaine accumulation and increased salt tolerance in rice seedlings | |||
| Journal | Volume | Year | Pages Concerned | |
| Biosci. Biotec. Biochem. | 60 | 1996 | 366-369 | |
| Author | Title of Article | |||
| T. Nakamura | Expression of betaine aldehyde dehydrogenase gene in rice, glycinebetaine nonaccumulator, and possible localization of its protein in peroxisomes | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant J. | 11 | 1997 | 1115-1120 | |
| Author | Title of Article | |||
| M. Nomura | Transgenically produced glycinebetaine protects ribulose 1, 5-bisphosphate carboxylase/oxygenase from inactivation in Synechococcus sp. PCC7942 under salt stress | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 39 | 1998 | 425-432 | |
| Author | Title of Article | |||
| T. Hibino | Molecular characterization of DnaK from the halotolerant cyanobacterium Aphanothece halophytica for ATPase, protein folding, and copper binding under various salinity conditions | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 40 | 1999 | 409-418 | |
| Author | Title of Article | |||
| N. Kaku | Expression of nhaAv gene encoding Na+/H+ antiporter from Vibrio alginolyticus in a freshwater cyanobacterium Synechococcus sp. PCC 7942 confers litium tolerance, but not sodium tolerance | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 40 | 1999 | 557-564 | |
| Author | Title of Article | |||
| Y. Tanaka | Salt tolerance of transgenic rice overexpressing yeast mitochondrial Mn-SOD in chloroplasts | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Science | 148 | 1999 | 131-138 | |
| Author | Title of Article | |||
| M. Sugino | Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica acquires resistance to salt stress in transgenic tobacco plants | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Science | 137 | 1999 | 81-88 | |
| Author | Title of Article | |||
| Y. Muramoto | Enhanced expression of a nuclease gene in leaves of barley plants under salt stress | |||
| Journal | Volume | Year | Pages Concerned | |
| Gene | 234 | 1999 | 315-321 | |
| Author | Title of Article | |||
| S. Kishitani | Compatibility of glycinebetaine in rice plants:evaluation using transgenic rice plants with a gene for peroxisomal betaine aldehyde dehydrogenase from barley | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Environ. | 23 | 2000 | 107-114 | |
| Author | Title of Article | |||
| H. Hoshida | Enhanced tolerance to salt stress in transgenic rice that overexpressed chloroplast glutamine synthetase | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 43 | 2000 | 103-111 | |
| Author | Title of Article | |||
| T. Hibino | Molecular cloning and functional characterization of two kinds of betaine aldehyde dehydrogenase in betaine accumulating mangrove Avicennia marina(Forsk. )Vierh | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 2001 | in press | ||
| Author | Title of Article | |||
| K. Ono | Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances the high-temperature tolerance o:tobacco during germination and early growth | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Science | 2001 | in press | ||
| Author | Title of Article | |||
| Y. Takeuchi | Induction and repair of damage to DNA in cucumber cotyledons irradiated with UV-B | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 37 | 1996 | 181-187 | |
| Author | Title of Article | |||
| Y. Takeuchi | The photorepair and photoisomerization of DNA lesions in etiolated cucumber cotyledons after irradiation by UV-B depends on wavelength | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 37 | 1998 | 745-750 | |
| Author | Title of Article | |||
| M. Hada | Action spectra of DNA photolyases for photorepair of cyclobutane pyrimidine dimers in sorghum and cucumber | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 41 | 2000 | 644-648 | |
| Author | Title of Article | |||
| Y. Takeuchi | Formation of DNA lesions in cucumber cotyledons exposed to solar UV radiation | |||
| Journal | Volume | Year | Pages Concerned | |
| Environ. Sci. | 13 | 2000 | 351-355 | |
| Author | Title of Article | |||
| T. Fujibe | Accumulation of pathogen-related proteins in tobacco leaves irradiated with UV-B | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 113 | 2000 | 387-394 | |
| Author | Title of Article | |||
| T. Berberich | Cycloheximide induces a subset of low temperature inducible genes in maize | |||
| Journal | Volume | Year | Pages Concerned | |
| Mol. Gen. Genet. | 254 | 1997 | 275-283 | |
| Author | Title of Article | |||
| T. Berberich | Two maize genes encoding plastidic ω-3 fatty acid desaturase and their differential expression to temperature | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 36 | 1998 | 297-306 | |
| Author | Title of Article | |||
| T. Kusano | Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein | |||
| Journal | Volume | Year | Pages Concerned | |
| Biochim. Biophys. Acta | 1395 | 1998 | 171-175 | |
| Author | Title of Article | |||
| K. Ito | Cold-inducible bZIP protein gene in radish root regulated by calcium-and cycloheximide-mediated signals | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Science | 142 | 1999 | 57-66 | |
| Author | Title of Article | |||
| T. Berberich | Involvement of a MAP kinase, ZmMPK5, in senescence and recovery from low-temperature-stress in maize | |||
| Journal | Volume | Year | Pages Concerned | |
| Mol. Gen. Genet. | 262 | 1999 | 534-542 | |
| Author | Title of Article | |||
| H. S. Yang | The darkness-induced and senescence-associated gene, Ntdin, encodes a protein functioning in molybdenum cofactor biosynthesis in tabacco plants | |||
| Journal | Volume | Year | Pages Concerned | |
| 2001 | submitted. | |||
| Author | Title of Article | |||
| H. S. Yang | Guard cell-and flower-specific expression of a tobacco gene, tbzF, encoding a bZIP-type transcriptional activator | |||
| Journal | Volume | Year | Pages Concerned | |
| 2001 | submitted | |||
| Author | Title of Article | |||
| I. Nishida | A second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, Cucurbita moschata cv. Shirogikuza, codes for an oleate-selective isozyme: molecular cloning and protein purification studies | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 41 | 2000 | 1381-1391 | |
| Author | Title of Article | |||
| Y. Fujiki | Multiple signaling pathways in gene expression during sugar starvation: pharmacological analysis of din gene expression in suspension-cultured cells of Arabidopsis | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 124 | 2000 | 1139-1147 | |
| Author | Title of Article | |||
| S. Yoshida | Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 42 | 2001 | 170-178 | |
| Author | Title of Article | |||
| Y. Fujiki | Dark-inducible genes from Arabidopsis thaliana are associated with leaf senescence and repressed by sugars. | |||
| Journal | Volume | Year | Pages Concerned | |
| Physiol. Plant. | 111 | 2001 | 345-352 | |
| Author | Title of Article | |||
| A. P. Tumbul | Analysis of the structure, substrate specificity and mechanism of the glycerol-3-phosphate 1-acyltransferase from squash (Cucurbita moschata) | |||
| Journal | Volume | Year | Pages Concerned | |
| Acta Cryst. | D57 | 2001 | 451-453 | |
| Author | Title of Article | |||
| M. K. Watahiki | The massugul mutation of Arabidopsis identified with failure of auxin-induced growth curvature of hypocotyl confers auxin insensitivity to hypocotyl and leaf | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 115 | 1997 | 419-426 | |
| Author | Title of Article | |||
| H. Hashimoto | Three more members of the Aux/IAA gene family from mung bean (Vigna radiata) hypocotyl (Accession Nos. AB004931, AB004932 and AB004933) (PGR 97-137) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 115 | 1997 | 315 | |
| Author | Title of Article | |||
| T. Kurata | Light-stimulated root elongation in Arabidopsis thaliana | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Physiol. | 151 | 1997 | 346-351 | |
| Author | Title of Article | |||
| T. Kurata | petitl, a conditional growth mutant of Arabidopsis defective in sucrose-dependent elongation growth | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 118 | 1998 | 793-801 | |
| Author | Title of Article | |||
| H. Hashimoto | An auxin down-regulated mRNA from mung bean (Vigna radiata) hypocotyl (Accession No. AB012110) is related to an aluminum-inducible mRNA in wheat root (PGR 98-094) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 117 | 1998 | 717 | |
| Author | Title of Article | |||
| M. Yamamoto | Differential effects of 1-naphthaleneacetic acid, indole-3-acetic acid and 2, 4-dichlorophenoxyacetic acid on the gravitropic response of roots in an auxin-resistant mutant of Arabidopsis, aux1 | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 39 | 1998 | 660-664 | |
| Author | Title of Article | |||
| M. Yamamoto | Effects of natural and synthetic auxins on the agravitropic growth habit of roots in auxin-resistant mutants of Arabidopsis, axr1 and axr4: Evidence for defects in the auxin influx mechanism of axr4 | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 112 | 1999 | 391-396 | |
| Author | Title of Article | |||
| H. Hashimoto | Isolation and expression of an elongation-dependent gene of mung bean (Vigna radiata) hypocotyl | |||
| Journal | Volume | Year | Pages Concerned | |
| Physiol. Plant. | 106 | 1999 | 224-231 | |
| Author | Title of Article | |||
| M. K. Watahiki, | The MSG1 and AXR1 genes of Arabidopsis are likely to act independently in growth-curvature responses of hypocotyls | |||
| Journal | Volume | Year | Pages Concerned | |
| Planta | 207 | 1999 | 362-369 | |
| Author | Title of Article | |||
| R. M. Harper | The NPH4 locus encodes the auxin response factor ARF7, a conditional regulator of differential growth in aerial Arabidopsis tissue | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell | 12 | 2000 | 757-770 | |
| Author | Title of Article | |||
| K. Fujihira | An agravitropic mutant of Arabidopsis, endodermal amyloplast-lessl, that lacks amyloplasts in hypocotyl endodermal cell layer | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 41 | 2000 | 1193-1199 | |
| Author | Title of Article | |||
| T. Fujibe | Accumulation of pathogenesis-related proteins in tobacco leaves irradiated with UV-B | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 113 | 2000 | 387-394 | |
| Author | Title of Article | |||
| S. Yoshida | Mechanism of adaptation to chilling temperatures in plants | |||
| Journal | Volume | Year | Pages Concerned | |
| Cryobiology and Cryotechnology | 42 | 1996 | 45-48 | |
| Author | Title of Article | |||
| M. Koike | Accumulation of 19-kDa plasma membrane polypeptide during induction of freezing tolerance in wheat suspension-cultured cells by abscisic acid | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 38 | 1997 | 707-716 | |
| Author | Title of Article | |||
| M. Murai | Vacuolar membrane lesions induced by a freeze-thaw cycle in protoplasts isolated from deacclimated tubers of Jerusalem artichoke (Helianthus tuberosus L. ) | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 39 | 1998 | 87-96 | |
| Author | Title of Article | |||
| M. Murai | Evidence for the cell wall involvement in temporal changes in freezing tolerance of Jerusalem artichoke (Helianthus tuberosus L. ) tubers during cold acclimation | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 39 | 1998 | 97-105 | |
| Author | Title of Article | |||
| S. Yoshida | A prefatory note on responses of plants to low temperature stress | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 112 | 1999 | 223-224 | |
| Author | Title of Article | |||
| C. Kuwabara | Abscisic acid-induced secretory protieins in suspnesion-cultured cell of winter wheat | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Cell Physiol. | 40 | 1999 | 184-191 | |
| Author | Title of Article | |||
| D. Takezawa | Elicitor and A23187-induced expression of WCK-1, a gene encoding mitogen-activated protein kinase in wheat | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 40 | 1999 | 921-933 | |
| Author | Title of Article | |||
| S. Yoshida | Alterations of intracellular pH in response to low temperature stresses | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 112 | 1999 | 225-236 | |
| Author | Title of Article | |||
| N. Ukaji | Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Physiol. | 120 | 1999 | 481-490 | |
| Author | Title of Article | |||
| M. Nagao | Akinete formation in Tribonema bombycinum Derbes et Solier (Xanthophyceae) in relation to freezing tolerance | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Plant Res. | 112 | 1999 | 163-174 | |
| Author | Title of Article | |||
| Y. Kawamura | Tissue specificity of E subunit isoforms of plant vacuolar H+- ATPase and existence of isotype enzymes | |||
| Journal | Volume | Year | Pages Concerned | |
| J. Biol. Chem. | 275 | 2000 | 6515-6522 | |
| Author | Title of Article | |||
| D. Takezawa | A rapid induction by elicitors of the mRNA encoding CCD-1, a 14 kDa Ca2+-binding protein in wheat cultured cells | |||
| Journal | Volume | Year | Pages Concerned | |
| Plant Mol. Biol. | 42 | 2000 | 807-817 | |
| Author | Title of Book | ||
| M. Otani | Biotechnology in Agriculture and Forestry | ||
| Publisher | Year | Pages | |
| Springer-Verlag | 2000 | in press | |
| Author | Title of Book | ||
| M. Otani | Biotechnology in Agriculture and Forestry | ||
| Publisher | Year | Pages | |
| Springer-Verlag | 2000 | in press | |
| Author | Title of Book | ||
| M. Otani | Transgenic Plants | ||
| Publisher | Year | Pages | |
| Maecel Dekker Inc. | 2000 | in press | |
| Author | Title of Book | ||
| K. Hotsubo | Plant Cold Hardiness | ||
| Publisher | Year | Pages | |
| Plenum Press | 1998 | 8 | |
| Author | Title of Book | ||
| N. Ukaji | Methods in Electron Microscopy and Cytochemistry | ||
| Publisher | Year | Pages | |
| Humana Press | 2000 | 14 | |
| Author | Title of Book | ||
| I. Nishida | Physiology, Biochemistry and Molecular Biology of Plant Lipids | ||
| Publisher | Year | Pages | |
| Kluwer Academic Publishers | 1997 | 3 | |
| Author | Title of Book | ||
| I. Nishida | Advances in Plant Lipid Research | ||
| Publisher | Year | Pages | |
| Universidad de Sevilla | 1998 | 4 | |