Summary of Research Project Results Under the JSPS FY2000
"Research for the future Program"
| 1.Research Institution | | Okazaki National Institute |
| |
| 2.Research Area | | Life Science |
| |
| 3.Research Field | | Organism Constructing Mechanisms |
| |
| 4.Term of Project | | FY1996〜FY2000 |
| |
| 5.Project Number | | 96L00407 |
| |
| 6.Title of Project | | Molecular mechanisms of reversible transformation of organelles in differentiation of higher plant cells |
7.Projetct Leader
| Name |
Institution,Department |
Title of Position |
| Mikio Nishimura |
Okazaki National Institute, Department of Cell Biology |
Professor |
8.Core Members
| Names |
Institution,Department |
Title of Position |
| Ikuko Hara-Nishimura |
Kyoto Univ., Graduate School of Science |
Professor |
9.Cooperating Researchers
| Names |
Institution,Department |
Title of Position |
| Tetsuko Noguchi |
Nara Women's Univ., Faculty of Science |
Professor |
| Makoto Hayashi |
Okazaki National Institute, Department of Cell Biology |
Associate Professor |
| Akira Kato |
Niigata Univ., Faculty of Science |
Associate Professor |
10.Summary of Research Results
In order to clarify the molecular medhanisms underlying reversible transformation of plant organelles, we analyzed the functional transformation of vacuoles and microbodies. The results are summarized as follows.
(1) Demonstration of a novel transport pathway of vacuolar proteins which is independent from Golgi-apparatus.
(2) Identification of a sorting receptor for vacuolar proteins.
(3) Molecular and functional characterization of vacuolar processing enzymes responsible for maturation of vacuolar proteins.
(4) Post-transcriptional regulation of matrix and membrane proteins of microbodies during the microbody transition.
(5) Isolation of peroxisome defective mutants of Arabidopsis and identification of these corresponding genes.
(6) Discovery of light-dependent alternative splicing in higher plants and characterization of the components in the splicing.
|
11.Key Words
(1)Differentiation of plant cells、(2)Differentiation of organelles、(3)Vacuole
(4)Transport vesicles、(5)Vacuolar processing enzyme、(6)Microbody transition
(7)Alternative splicing、(8)Peroxisome defective mutant
12.References
[Reference Articles]
| Author |
Title of Article |
| Hayashi, M. |
Pumpkin hydroxypyruvate reductases with and without a putative C-terminal signal for targeting to microbodies may be produced by alternative splicing. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Mol. Biol. |
30 |
1996 |
183-189 |
| Author |
Title of Article |
| Yamaguchi, K. |
cDNA cloning for thylakoid-bound ascorbate peroxidase in pumpkin and its charac-terization. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
37 |
1996 |
405-409 |
| Author |
Title of Article |
| Hayashi, M |
Transport of chimeric proteins containing carboxy-terminal targeting signal into microbodies of transgenic Arabidopsis |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant J. |
10 |
1996 |
225-234 |
| Author |
Title of Article |
| Kato, A. |
cDNA cloning and expression of a gene for 3-ketoacyl-CoA thiolase in pumpkin cotyledons. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Mol. Biol. |
31 |
1996 |
843-852 |
| Author |
Title of Article |
| Kato, A. |
Targeting and processing of a chimeric protein with the amino-terminal presequence of the precursor to glyoxysomal citrate synthase. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell |
8 |
1996 |
1601-1611 |
| Author |
Title of Article |
| Koumoto, Y. |
Isolation and characterization of a cDNA encoding mitochondrial chaperonin 10 from Arabidopsis thaliana by functional complementation of E coli mutant |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant J. |
10 |
1996 |
1119-1125 |
| Author |
Title of Article |
| Mano, S. |
cDNA cloning and expression of a gene for isocitrate lyase in pumpkin cotyledons. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
37 |
1996 |
941-948 |
| Author |
Title of Article |
| Esaka, M. |
cDNA cloning and differential gene expression of three catalases in pumpkin. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Mol. Biol. |
33 |
1997 |
141-155 |
| Author |
Title of Article |
| Hatano, K. |
A rapid increase in the level of bindig protein (BiP) is accompanied by synthesis and degradation of storage proteins in pumpkin cotyledons. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
38 |
1997 |
344-351 |
| Author |
Title of Article |
| Inoue, K. |
Heterologous expression and subcellular localization of pumpkin seed tonoplast intrinsic (TIP) in yeast cells |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
38 |
1997 |
366-370 |
| Author |
Title of Article |
| Mano, S. |
Hydroxypyruvate reductase with a carboxy-terminal targeting signal to microbodies is expressed in Arabidopsis. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
38 |
1997 |
449-455 |
| Author |
Title of Article |
| Hiraiwa, N. |
An aspartic proteinase is involved in the breakdown of polypeptides of storage proteins in protein-storage vacuoles of plants. |
| Journal |
Volume |
Year |
Pages Concerned |
| Eur. J. Biochem. |
246 |
1997 |
133-141 |
| Author |
Title of Article |
| Hayashi, M. |
Changes in targeting efficiencies of proteins to plant microbodies caused by amino acid substitution in the carboxy-terminal tripeptide. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
38 |
1997 |
759-768 |
| Author |
Title of Article |
| Mano, S. |
Stromal and thylakoid-bound ascorbate peroxidases are produced by alternative splicing in pumpkin. |
| Journal |
Volume |
Year |
Pages Concerned |
| FEBS Lett. |
413 |
1997 |
21-26 |
| Author |
Title of Article |
| Minami, Y. |
β-glucosidase in the Indigo plant: intracellular localization and tissue specific expression in leaves. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
38 |
1997 |
1069-1074 |
| Author |
Title of Article |
| Hiraiwa, N. |
Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant J. |
12 |
1997 |
819-829 |
| Author |
Title of Article |
| Nii, N. |
Changes in carbohydrate and ribulose bisphosphate carboxylase contents in peach leaves after applications of different amounts of nitrogen fertilizer |
| Journal |
Volume |
Year |
Pages Concerned |
| J. Jap. Soc. Hort. Sci. |
66 |
1997 |
505-511 |
| Author |
Title of Article |
| Sato, M. |
The AtVAM3 encodes a syntaxin-related molecule implicated in the vacuolar assembly in Arabidopsis thaliana. |
| Journal |
Volume |
Year |
Pages Concerned |
| J. Biol. Chem. |
272 |
1997 |
24530-24535 |
| Author |
Title of Article |
| Nozue, M. |
Expression of a vacuolar protein (VP24) in anthocyanin-producing cells of sweet potato in suspension culture. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Physiol. |
115 |
1997 |
1065-1072 |
| Author |
Title of Article |
| Shimada, T. |
A pumpkin 72-kDa membrane protein of precursor accumulating vesicles have characteristics for a vacuolar sorting receptor. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
38 |
1997 |
1414-1420 |
| Author |
Title of Article |
| Kato, A. |
Glyoxysomal malate dehydrogenase in pumpkin: cloning of a cDNA and functional analysis of its presequence. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
39 |
1998 |
186-195 |
| Author |
Title of Article |
| Hayashi, M. |
2, 4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects on glyoxysomal b-oxidation. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell |
10 |
1998 |
183-195 |
| Author |
Title of Article |
| Hayashi, H. |
Molecular characterization of a glyoxysomal long-chain acyl-CoA oxidase that is synthesized as a precursor of higher molecular mass in pumpkin. |
| Journal |
Volume |
Year |
Pages Concerned |
| J. Biol. Chem. |
273 |
1998 |
8301-8307 |
| Author |
Title of Article |
| Hara-Nishimura, I. |
Transport of storage proteins to protein-storage vacuoles is mediated by large precursor-accumulating vesicles. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell |
10 |
1998 |
825-836 |
| Author |
Title of Article |
| Yamada, K. |
Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor protein by vacuolar processing enzyme. |
| Journal |
Volume |
Year |
Pages Concerned |
| J. Biol. Chem. |
274 |
1999 |
2563-2570 |
| Author |
Title of Article |
| Hayashi, M. |
Induction of precursor-accumulating vesicles by expression of chimeric genes consisting of pumpkin 2S albumin and phosphinothricin acetyltransferase. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
40 |
1999 |
263-272 |
| Author |
Title of Article |
| Mano, S. |
Light regulates alternative splicing of hydroxypyruvate reductase in pumpkin. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant J. |
17 |
1999 |
309-320 |
| Author |
Title of Article |
| Koumoto, Y. |
Chloroplast Cpn20 forms a tetrameric structure in Arabidopsis thaliana. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant J. |
17 |
1999 |
467-477 |
| Author |
Title of Article |
| Hiraiwa, N. |
Vacuolar processing enzyme is activated self-catalytically by sequential removal of the C-terminal and N-terminal propeptides. |
| Journal |
Volume |
Year |
Pages Concerned |
| FEBS Lett. |
447 |
1999 |
213-216 |
| Author |
Title of Article |
| Hayashi, H. |
A novel oxidase that catalyzes oxidation of short-chain acyl CoA in plant peroxi-somes. |
| Journal |
Volume |
Year |
Pages Concerned |
| J. Biol. Chem. |
274 |
1999 |
12715-12721 |
| Author |
Title of Article |
| Kato, A. |
Oligomeric proteins contatining N-terminal targeting signals are imported into peroxisomes in transgenic Arabidopsis. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
40 |
1999 |
586-591 |
| Author |
Title of Article |
| Kinoshita, T. |
Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescene and under various stressd conditions. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant J. |
19 |
1999 |
43-53 |
| Author |
Title of Article |
| Miyagishima, S. |
Microbody proliferation and segregation cycle in the single microbody-alga Cyanidioschyzon merolae. |
| Journal |
Volume |
Year |
Pages Concerned |
| Planta |
208 |
1999 |
326-336 |
| Author |
Title of Article |
| De Bellis L. |
Purification and characterization of pumpkin long-chain acyl-CoA oxidase. |
| Journal |
Volume |
Year |
Pages Concerned |
| Physiol. Plant. |
106 |
1999 |
170-176 |
| Author |
Title of Article |
| Takemoto, D. |
Molecular cloning of a defense-response-related cytochrome P450 gene from tobacco using a fungal elicitor. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
40 |
1999 |
1232-1242 |
| Author |
Title of Article |
| Ali, K. |
Levels of nonstructural carbohydrate in leaves and roots and some characteristics of chloro-plasts after application of different amounts of nitrogen fertilizer to peach seedlings. |
| Journal |
Volume |
Year |
Pages Concerned |
| J. Jap. Soc. Hort. Sci. |
68 |
1999 |
739-745 |
| Author |
Title of Article |
| Minami, Y. |
Tissue and intracellular localization of indican and the purification and characterization of indican synthase from Indigo plants |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
41 |
2000 |
218-225 |
| Author |
Title of Article |
| Takemoto, D. |
Isolation of gene for EIR, an elicitor inducible receptor like protein, from tobacco by differential display. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
41 |
2000 |
458-464 |
| Author |
Title of Article |
| De Bellis, L. |
Purification and characterization of a novel pumpkin short-chain acyl-CoA oxidase which structure resembles acyl-CoA dehydrogenase. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Physiol. |
123 |
2000 |
327-334 |
| Author |
Title of Article |
| Mitsuhashi, N. |
Characterization of organelles in the vacuolar-sorting pathway by visualization with GFP in tobacco BY-2 cells. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
41 |
2000 |
993-1001 |
| Author |
Title of Article |
| Hayashi, M. |
AtPex14 maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes. |
| Journal |
Volume |
Year |
Pages Concerned |
| EMBO J. |
19 |
2000 |
5701-5710 |
| Author |
Title of Article |
| Yamaguchi, K. |
Reduction to below threshold levels of glycolate oxidase activities in transgenic tobacco enhances photoinhibition during irradiation. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
41 |
2000 |
1397-1406 |
| Author |
Title of Article |
| Xu, W. |
Detection and characterization of a 36-kDa peptide in C-terminal region of a 24-kDa vacuolar protein (VP24) precursor in anthocyanin-producing sweet potato cells in suspension culture. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Sci. |
160 |
2000 |
121-128 |
| Author |
Title of Article |
| Nito, K. |
Pumpkin peroxisomal ascorbate peroxidase is localized on peroxisomal membranes and unknown membranous structures. |
| Journal |
Volume |
Year |
Pages Concerned |
| Plant Cell Physiol. |
42 |
2001 |
20-27 |
| Author |
Title of Article |
| Hayashi-Ishimaru, Y. |
Glyoxysome-spherosome interaction in etiolated cotyledons of Arabidopsis Pedl mutant that has defect on a thiolase gene of fatty acid b-oxidation cycle. |
| Journal |
Volume |
Year |
Pages Concerned |
| Protoplasma |
|
2001 |
in press |
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