Summary of Research Project Results Under the JSPS FY2000
"Research for the future Program"



1.Research Institution HOKKAIDO UNIVERSITY
 
2.Research Area Life Sciences
 
3.Research Field Organism Constructing Mechanisms
 
4.Term of Project FY1996〜FY2000
 
5.Project Number 96L00403
 
6.Title of Project Study of molecular switches for the reproductive development in plants.

7.Projetct Leader
Name Institution,Department Title of Position
KOMEDA, Yoshibumi Hokkaido Univ., Graduate School of Science Professor

8.Core Members

Names Institution,Department Title of Position
NISHITANI, Kazuhiko Tohoku Univ., Graduate School of Science Professor
GOTO, Koji Research Institute for biological Sciences, Molecular Genetics Laboratory Lab Head
ARAKI, Takashi Kyoto Univ., Graduate School of Science Assistant Pro.

9.Cooperating Researchers

Names Institution,Department Title of Position
KATO, Hidetoshi Kyoto Univ., Institute for Chemical Research Research Asso.
KURATA, Tetuya Hokkaido Univ., Graduate School of Science Research Asso.
Takahashi-Hanzawa Yoshie Hokkaido Univ., Graduate School of Science Research Asso.

10.Summary of Research Results

The TERMINAL FLOWER 1 (TFL1) gene of Arabidopsis serves a key function in the development of the shoot apical meristem (SAM). The specific expression of TFL1 transcripts in the subapical region of the SAM suggests that TFL1 functions non-cell-autonomously and that TFL1 function may involve cell-cell communication. We demonstrate that the TFL1 protein is transmitted from the subapical region to the epidermis, and suggest that this protein trafficking is regulated during the developmental phase of the SAM. Our results suggest that TFL1 protein trafficking gives rise to the interlayer signaling responsible for the coordination of the distinct layers of the meristem.
The L1-layer specific genes, PDF1 and PDF2 were isolated and studies. The PDF1 has the L1-box cis regulator.
The PDF1 gene has the HD-GL2 class Homeo domain. This is suggested to be regulator of PDF1 gene by gel-shift assay. The transgenic plants carrying over-expression and co-suppressed PDF2 gene were isolated and studies. They showed various flowering phenotypes, which suggested the roles of L1-layer for flowering.
Molecular genetic analysis of Arabidopsis FT locus has elucidated its role in integrating three genetic pathways regulating the floral transition. CONSTANS mediates signals from the photoperiod pathway by directly activating FT transcription. The autonomous and vernalization pathways act through FLOWERING LOCUS C which encodes a putative negative regulator of FT expression. FT acts in parallel with another pathway integrator, LEAFY, and in an antagonistic manner with its homolog, TERMINAL FLOWER1.
We investigated functions of individual members of a gene family which encode endoxyloglucan transferases. What we found is as follows: 1) This multi-gene family consists of 33 genes in Arabidopsis. 2) individual genes exhibit spatially and physiologically distinct expression profiles, some members being involved in construction of cell plate and others in the primary and secondary cell walls. 3) EXGT-A3 gene disruptant showed morphological changes in the shoot of Arabidopsis.

11.Key Words

(1)PDF1、(2)PDF2、(3)Flower morphogenesis
(4)Flowering-time genes、(5)cell to cell communication、(6)Chromatin Assembly Factor-1
(7)TFL1、(8)cell wall、(9)endoxyloglucan transferase

12.References

[Reference Articles]
Author Title of Article
Hanzawa, Y. ACL5: an Arabidopsis gene required for the internodal elongation after flowering.
Journal Volume Year Pages Concerned
The Plant Journal 12(4) 1997 863-874

Author Title of Article
Yokoyama, R. The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia
Journal Volume Year Pages Concerned
The Plant Journal 15(3) 1998 301-310

Author Title of Article
Komeda, Y. Development of Inflorescences in Arabidopsis thaliana
Journal Volume Year Pages Concerned
Journal of Plant Research (JPR Symposium) 111(1102) 1998 283-288

Author Title of Article
Abe, M. Cloning and Characterization of an L1 Layer-specific Gene in Arabidopsis thaliana.
Journal Volume Year Pages Concerned
Plant and Cell Physiology 40(6) 1999 571-580

Author Title of Article
Akamatsu, T. Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis.
Journal Volume Year Pages Concerned
Plant Physiology 121(3) 1999 715-721

Author Title of Article
Kato, A. Isolation and analysis of cDNA within a 300 kb Arabidopsis thaliana genomic region located around the 100 map unit of chromosome 1.
Journal Volume Year Pages Concerned
Gene 239 1999 309-316

Author Title of Article
高橋 卓 熱ショック応答の分子機構
Journal Volume Year Pages Concerned
蛋白質核酸酵素 44(15) 1999 39-44

Author Title of Article
Suzuki, M. An RNA-binding protein, AtRBP1, is expressed in actively proliferative regions in Arabidopsis thaliana.
Journal Volume Year Pages Concerned
Plant and Cell Physiology 41(3) 2000 282-288

Author Title of Article
Kuwahara, A. Isolation and characterization of copia-type retrotransposons in Arabidopsis thaliana.
Journal Volume Year Pages Concerned
Gene 244 2000 127-136

Author Title of Article
Matsuhara, S. Heat-shock tagging: a simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions.
Journal Volume Year Pages Concerned
The Plant Journal 22(1) 2000 79-86

Author Title of Article
Hanzawa, Y. ACAULIS5, an Arabidopsis gene required for stem elongation, encodes a spermine synthase.
Journal Volume Year Pages Concerned
The EMBO Journal 19(16) 2000 4248-4256

Author Title of Article
Ohtake, Y. Salicylic acid induces the expression of a number of receptor-like kinase genes in Arabidopsis thaliana.
Journal Volume Year Pages Concerned
Plant and Cell Physiology 41(9) 2000 1038-1044

Author Title of Article
Abe, M. Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein.
Journal Volume Year Pages Concerned
The Plant Journal   2001 in press

Author Title of Article
Nishitani, K. The role of endoxyloglucan transferase in the organization of plant cell walls.
Journal Volume Year Pages Concerned
International Review of Cytology 173 1997 157-206

Author Title of Article
Nishitani, K. Implication of xyloglucan related protein (XRP) family in regulation of plant growth and development.
Journal Volume Year Pages Concerned
Trends in Glycoscience and Glycotechnology 9 1997 233-234

Author Title of Article
西谷 和彦 アポプラストの枠組みの構築
Journal Volume Year Pages Concerned
化学と生物 35 1997 790-797

Author Title of Article
Nishitani, K. Construction and restructuring of the cellulose-xyloglucan framework in the apoplast as mediated by the xyloglucan-related protein family - A hypothetical scheme.
Journal Volume Year Pages Concerned
J. Plant Res. 111 1998 159-166

Author Title of Article
Takano M. Endoxyloglucan transferase cDNA isolated from pea roots and its fluctuating expression in hydrotropically responding roots.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 40(2) 1999 135-142

Author Title of Article
Ito, H. Visualization of EXGT-mediated molecular grafting activity by means of a fluorescently labeled xyloglucan oligomer.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 40 1999 1172-1176

Author Title of Article
Yokoyama, R. Functional diversity of xyloglucan-related proteins and its implications in the cell wall dynamics in plants.
Journal Volume Year Pages Concerned
Plant bioloby 2 2000 598-604

Author Title of Article
Yokoyama, R. Endoxyloglucan transferase is localized both in the cell plate and in the secretory pathway destined for the apoplast in tobacco cells.
Journal Volume Year Pages Concerned
Plant Cell Physiol. 42(3) 2001 in press

Author Title of Article
Goto, K. Molecular and genetic analyses of flower homeotic genes of Arabidopsis.
Journal Volume Year Pages Concerned
Journal of Biosciences. 21 1996 369-378

Author Title of Article
Goto, K. Molecular mechanisms of petal and stamen development in Arabidopsis.
Journal Volume Year Pages Concerned
Journal of Reproduction and Development. 43 1997 19-20

Author Title of Article
工藤 光子 花の器官の並ぶメカニズム―ABCモデル―
Journal Volume Year Pages Concerned
遺伝 51(4) 1997 27-33

Author Title of Article
Kato, H CENTRORADIALIS/TERMINAL FLOWER 1 Gene Homolog is Conserved in N. tabacum, a Determinate Inflorescence Plant.
Journal Volume Year Pages Concerned
Journal of Plant Research (JPR Symposium) 111 1998 289-294

Author Title of Article
Sawa, S. FILAMENTOUS FLOWER, a meristem and organ identity gene of Arabidopsis, encodes a protein with a zinc finger and a HMG-related domains.
Journal Volume Year Pages Concerned
Genes & Development 13 1999 1079-1088

Author Title of Article
Kobayashi, Y. A pair of related genes with antagonistic roles in mediating flowering signals.
Journal Volume Year Pages Concerned
Science 286 1999 1960-1962

Author Title of Article
Honma, T. The Arabidopsis floral homeotic gene PISTILLATA is regulated by discrete cis-elements responsive to induction and maintenance signals.
Journal Volume Year Pages Concerned
Development 127 2000 2021-2030

Author Title of Article
Honma, T. Complexes of MADS-box proteins are sufficient to convert leaves into floral organs.
Journal Volume Year Pages Concerned
Nature 409 2001 525-529

Author Title of Article
Minida, N. Functional divergence of the TFL1-like gene family in Arabidopsis revealed by characterization of a novel member, ATC.
Journal Volume Year Pages Concerned
Genes to Cells   2001 in press

Author Title of Article
Okada, K. The genetic basis of phenotype expression in plants.
Journal Volume Year Pages Concerned
Plant Species Biol. 11 1996 115-139

Author Title of Article
Araki, T. [in Japanese] Genes directing flowering and early steps of floral morphogenesis.
Journal Volume Year Pages Concerned
Idenn [Heredity] 51(4) 1997 19-26

Author Title of Article
Araki, T. [in Japanese] Natural history of flowering.
Journal Volume Year Pages Concerned
Biohistory 5(1) 1997 10-15

Author Title of Article
Araki, T. [in Japanese] Genetic control of flowering: transition from vegetative to reproductive growth.
Journal Volume Year Pages Concerned
Protein, Nucleic Acid, and Enzyme 43(4) 1998 583-591

Author Title of Article
Araki, T. The flowering-time gene FT and regulation of flowering in Arabidopsis.
Journal Volume Year Pages Concerned
Journal of Plant Research (JPR Symposium) 111 1998 277-281

Author Title of Article
Taoka, K-i. Identification of three kinds of mutually related composite elements conferring S phase-specific transcriptional activation.
Journal Volume Year Pages Concerned
Plant Journal 18(6) 1999 611-623

Author Title of Article
Sakamoto, H. Expression of a subset of the Arabidopsis Cys2/His2-type zinc finger protein gene family under water stress.
Journal Volume Year Pages Concerned
Gene 248(1/2) 2000 23-32

Author Title of Article
Kaya, H. hosoba toge toge, a syndrome caused by a large chromosomal deletion associated with a T-DNA insertion in Arabidopsis.
Journal Volume Year Pages Concerned
Plant and Cell Physiology 41(9) 2000 1055-1066

Author Title of Article
Kaya, H. FASCIATA genes for chromatin assembly factor-1 in Arabidopsis maintain the cellular organization of apical meristems.
Journal Volume Year Pages Concerned
Cell 104(1) 2001 131-142

Author Title of Article
Araki, T. Transition from vegetative to reproductive phase.
Journal Volume Year Pages Concerned
Current Opinion in Plant Biology 4(1) 2001 63-68

Author Title of Article
Araki, T. [in Japanese] Integration of multiple genetic pathways controlling the floral transition.
Journal Volume Year Pages Concerned
Kagaku to Seibutsu [Chemistry and Biology] 38(12) 2000 826-829

Author Title of Article
Kaya, H. [in Japanese] A role of CAF-1 in post-embryonic development in Arabidopsis.
Journal Volume Year Pages Concerned
Cell Technology 20(2) 2001 176-177

[Reference Books]
Author Title of Book
Kumiki Inaba-Higano [in Japanese] Induction of mutations (Manual of the experiments of plant growth regulation. )
Publisher Year Pages
Ed. : Shigeo Yoshida (The Society of Plant Growth Regulation of Plants) 1997 199

Author Title of Book
Yoshibumi Komeda [in Japanese] Isolation of genes by DNA-tagging (Manual of the experiments of plant growth regulation. )
Publisher Year Pages
Ed. : Shigeo Yoshida (The Society of Plant Growth Regulation of Plants) 1997 199

Author Title of Book
Yoshibumi Komeda [in Japanese] Isolation of genes by using mutants (Manual of the experiments of plant growth regulation. )
Publisher Year Pages
Ed. : Shigeo Yoshida (The Society of Plant Growth Regulation of Plants) 1997 199

Author Title of Book
半澤 芳恵 茎伸長の分子機構 (Genetic control of internode elongation.) 「新版 植物の形を決める分子機構」 (岡田清孝ほか編)
Publisher Year Pages
秀潤社 2000 292

Author Title of Book
倉田 哲也 花芽と花序の分化 (花・性と生殖の分子生物学・編:日向康吉)
Publisher Year Pages
学会出版センター 2001 258

Author Title of Book
西谷 和彦 細胞工学別冊 植物のタンパク質実験プロトコール
Publisher Year Pages
秀潤社 1998 206

Author Title of Book
西谷 和彦 エンド型キシログルカン転移酵素と細胞壁構築 (植物ホルモンと細胞壁)
Publisher Year Pages
学会出版センター 1998 190

Author Title of Book
Nishitani, K. Endoxyloglucan transferases (Glycoenzymes)
Publisher Year Pages
Japanese Scientific Societies Press 2000 263

Author Title of Book
後藤 弘爾 組織へのin situハイブリダイゼーション。 (細胞工学別冊 植物細胞工学シリーズ4:モデル植物の実験プロトコール)
Publisher Year Pages
秀潤社 1996 260

Author Title of Book
後藤 弘爾 花の形を決める遺伝子 (植物の生長 遺伝子から何が見えるか)
Publisher Year Pages
クバプロ 1998 169(86-94)

Author Title of Book
後藤弘爾 モデル生物の分子生物学…アラビドプシス(シロイヌナズナ) (分子生物学)
Publisher Year Pages
東京化学同人 1999 326(287-292)

Author Title of Book
後藤弘爾 遺伝子の発現をみる -in situハイブリダイゼーション- (モデル植物ラボマニュアル 分子遺伝学・分子生物学的実験法)
Publisher Year Pages
シュプリンガー・フェアラーク東京株式会社 2000 275(204-217)

Author Title of Book
Araki, T. [in Japanese] A Protocol Book for Model Plant Species, Arabidopsis and Rice. (Plant Cell Technology Series 4)
Publisher Year Pages
Shujunsha 1996 260(109-113)

Author Title of Book
Araki, T. [in Japanese] Molecular Mechanisms of Controlling Multicellular Organization of Plants. (Plant Cell Technology Series 12)
Publisher Year Pages
Shujunsha 2000 292(138-149)

Author Title of Book
荒木 崇 遺伝子導入のための設備(シロイヌナズナ)(モデル植物ラボマニュアル 分子遺伝学・分子生物学的実験法・編:岩渕雅樹・岡田清孝・島本 功)
Publisher Year Pages
シュプリンガー・フェアラーク東京株式会社 2000 275(29-30)

Author Title of Book
荒木 崇 トランスジェニック植物をつくる(シロイヌナズナ)(モデル植物ラボマニュアル 分子遺伝学・分子生物学的実験法・編:岩渕雅樹・岡田清孝・島本 功)
Publisher Year Pages
シュプリンガー・フェアラーク東京株式会社 2000 275(99-109)

Author Title of Book
荒木 崇 遺伝子をクローニングする・タギング法・(シロイヌナズナ). (モデル植物ラボマニュアル分子遺伝学・分子生物学的実験法・編:岩渕雅樹・岡田清孝・島本 功)
Publisher Year Pages
シュプリンガー・フェアラーク東京株式会社 2000 275(162-168)

Author Title of Book
Kobayashi, Y. [in Japanese] Regulation of Cell Division in Plants. (Plant Cell Technology Series 13)
Publisher Year Pages
Shujunsha 2000 188

Author Title of Book
Araki, T. [in Japanese] Flower:Molecular Biology of Sex Expression and Sexual Reproduction in Plants.
Publisher Year Pages
Japan Scientific Societies Press 2001 258 (41-51)


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