Summary of Research Project Results Under the JSPS FY2000
"Research for the future Program"



1.Research Institution Okazaki National Research Institutes
 
2.Research Area Life Sciences
 
3.Research Field Organism Constructing Mechanisms
 
4.Term of Project FY1996〜FY2000
 
5.Project Number 96L00401
 
6.Title of Project Molecular and Cellular Mechanisms of Sex differentiation in Fish

7.Projetct Leader
Name Institution,Department Title of Position
Yoshitaka Nagahama Okazaki Natl. Res. Inst., Natl. Inst. Basic Biol. Professor

8.Core Members

Names Institution,Department Title of Position
Akihiro Shima Univ. Tokyo, Integrated Biosciences Professor
Kenjiro Ozato Nagoya Univ. Bioscience Center Professor

9.Cooperating Researchers

Names Institution,Department Title of Position
Yoshikuni, M. Okazaki Natl. Res. Inst., NIBB Associate Prof.
Kobayashi, T. Okazaki Natl. Res. Inst., NIBB Assist. Prof.
Wakamatsu, Y. Nagoya Univ., Bioscience Center Associate Prof.

10.Summary of Research Results

In this project, we have investigated the molecular and cellular mechanisms of sex determination and differentiation in teleost fish. The results of this project are as follows. 1) The estrogen/estrogen receptor system plays a critical role in ovarian differentiation in tilapia, 2) The androgen/androgen receptor system does not seem to play a role in testicular differentiation. Instead, the expression of DMRT1 gene in Sertoli cells is critical for testicular differentiation, 3) The sex-determining gene (PG17) of medaka was identified using positional cloning and termed osu, 4) Fertile and diploid nuclear transplants were successfully generated using embryonic cells as donors in medaka, 5) Medaka transgenic lines were generated with green fluorescent protein fluorescence controlled by the regulatory regions of the olvas gene in the germ cells, 6) See-through medaka (transparent throughout life) and Qurt strain (sex can be predicted in embryos two days after fertilization) were produced, and 7) The loss of aromatase is a permanent and critical step in the cascade of sex change (from female to male) in the wrasse.

11.Key Words

(1)sex differentiation、(2)sex determination、(3)gonads
(4)Fish、(5)medaka、(6)tilapia
(7)nuclear transplantation、(8)sex steroids、(9)DMRT1

12.References

[Reference Articles]
Author Title of Article
Wada, H. et al. Sex-linked inheritance of the if locus in the madako fish coryzias latipes
Journal Volume Year Pages Concerned
Zool. Sci. 15 1998 123-126

Author Title of Article
Ikeuchi, T. et al. c DNA cloning of a novel androgen receptor subtupe
Journal Volume Year Pages Concerned
g. Biol. Chem. 274 1999 25205-25209

Author Title of Article
Guan, G. et al. Sexually dimorphic expression of DM(doublsex/Mab-3)-domain genes during gonadal differentian・・・
Journal Volume Year Pages Concerned
Biochem. Biophys. Res. Covn. 272 2000 662-666

Author Title of Article
Tanaka, M. et al. Establishment of medaka(oriyzioslabpes)transgenic lines urth the expression of GFP fluorescence・・・
Journal Volume Year Pages Concerned
Proc. Natl. Acad. Sci. USA 98 2001 2544-2549

Author Title of Article
Wakamatsu, Y. et al. Fertile and diploid nuclear transptation deriued from embryonic cells of medaka, Orggies latipes
Journal Volume Year Pages Concerned
Proc. Natl. Acad. Sci. USA 98 2001 1071-1076

Author Title of Article
Wakamatsu, Y. et al. The see-throagh medaka : A fish model that is transporent throughout life
Journal Volume Year Pages Concerned
Proc. Natl. Acad. Sci. USA 98 2001 10046-10050

[Reference Books]
Author Title of Book
Y. Nagahama, T. Todo Fish DNA : A Molewlar Genetic Approach
Publisher Year Pages
Edited by T. Aoki, F. Takashima & T. Hirano 1997 350-378

Author Title of Book
K. Ozato & Y. Wakamatsu Fish DNA : A Molewlar Genetic Apprach
Publisher Year Pages
Edited by T. Aoki, F. Takashima & T. Hirano 1997 63-79


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