| 1.Research Institution | Okazaki National Research Institutes | |
| 2.Research Area | Life Sciences | |
| 3.Research Field | Organism Constructing Mechanisms | |
| 4.Term of Project | FY1996〜FY2000 | |
| 5.Project Number | 96L00401 | |
| 6.Title of Project | Molecular and Cellular Mechanisms of Sex differentiation in Fish |
| Name | Institution,Department | Title of Position |
| Yoshitaka Nagahama | Okazaki Natl. Res. Inst., Natl. Inst. Basic Biol. | Professor |
8.Core Members
| Names | Institution,Department | Title of Position |
| Akihiro Shima | Univ. Tokyo, Integrated Biosciences | Professor |
| Kenjiro Ozato | Nagoya Univ. Bioscience Center | Professor |
9.Cooperating Researchers
| Names | Institution,Department | Title of Position |
| Yoshikuni, M. | Okazaki Natl. Res. Inst., NIBB | Associate Prof. |
| Kobayashi, T. | Okazaki Natl. Res. Inst., NIBB | Assist. Prof. |
| Wakamatsu, Y. | Nagoya Univ., Bioscience Center | Associate Prof. |
10.Summary of Research Results
|
In this project, we have investigated the molecular and cellular mechanisms of sex determination and differentiation in teleost fish. The results of this project are as follows. 1) The estrogen/estrogen receptor system plays a critical role in ovarian differentiation in tilapia, 2) The androgen/androgen receptor system does not seem to play a role in testicular differentiation. Instead, the expression of DMRT1 gene in Sertoli cells is critical for testicular differentiation, 3) The sex-determining gene (PG17) of medaka was identified using positional cloning and termed osu, 4) Fertile and diploid nuclear transplants were successfully generated using embryonic cells as donors in medaka, 5) Medaka transgenic lines were generated with green fluorescent protein fluorescence controlled by the regulatory regions of the olvas gene in the germ cells, 6) See-through medaka (transparent throughout life) and Qurt strain (sex can be predicted in embryos two days after fertilization) were produced, and 7) The loss of aromatase is a permanent and critical step in the cascade of sex change (from female to male) in the wrasse. |
11.Key Words
(1)sex differentiation、(2)sex determination、(3)gonads
(4)Fish、(5)medaka、(6)tilapia
(7)nuclear transplantation、(8)sex steroids、(9)DMRT1
12.References
| Author | Title of Article | |||
| Wada, H. et al. | Sex-linked inheritance of the if locus in the madako fish coryzias latipes | |||
| Journal | Volume | Year | Pages Concerned | |
| Zool. Sci. | 15 | 1998 | 123-126 | |
| Author | Title of Article | |||
| Ikeuchi, T. et al. | c DNA cloning of a novel androgen receptor subtupe | |||
| Journal | Volume | Year | Pages Concerned | |
| g. Biol. Chem. | 274 | 1999 | 25205-25209 | |
| Author | Title of Article | |||
| Guan, G. et al. | Sexually dimorphic expression of DM(doublsex/Mab-3)-domain genes during gonadal differentian･･･ | |||
| Journal | Volume | Year | Pages Concerned | |
| Biochem. Biophys. Res. Covn. | 272 | 2000 | 662-666 | |
| Author | Title of Article | |||
| Tanaka, M. et al. | Establishment of medaka(oriyzioslabpes)transgenic lines urth the expression of GFP fluorescence･･･ | |||
| Journal | Volume | Year | Pages Concerned | |
| Proc. Natl. Acad. Sci. USA | 98 | 2001 | 2544-2549 | |
| Author | Title of Article | |||
| Wakamatsu, Y. et al. | Fertile and diploid nuclear transptation deriued from embryonic cells of medaka, Orggies latipes | |||
| Journal | Volume | Year | Pages Concerned | |
| Proc. Natl. Acad. Sci. USA | 98 | 2001 | 1071-1076 | |
| Author | Title of Article | |||
| Wakamatsu, Y. et al. | The see-throagh medaka : A fish model that is transporent throughout life | |||
| Journal | Volume | Year | Pages Concerned | |
| Proc. Natl. Acad. Sci. USA | 98 | 2001 | 10046-10050 | |
| Author | Title of Book | ||
| Y. Nagahama, T. Todo | Fish DNA : A Molewlar Genetic Approach | ||
| Publisher | Year | Pages | |
| Edited by T. Aoki, F. Takashima & T. Hirano | 1997 | 350-378 | |
| Author | Title of Book | ||
| K. Ozato & Y. Wakamatsu | Fish DNA : A Molewlar Genetic Apprach | ||
| Publisher | Year | Pages | |
| Edited by T. Aoki, F. Takashima & T. Hirano | 1997 | 63-79 | |