| 1.Research Institution | Waseda University | |
| 2.Research Area | Life Sciences | |
| 3.Research Field | Cellular Signaling | |
| 4.Term of Project | FY1996〜FY2000 | |
| 5.Project Number | 96L00310 | |
| 6.Title of Project | Signaling Mechanism in Neuronal System |
| Name | Institution,Department | Title of Position |
| Yoshioka, Tohru | Waseda University, School of Human Sciences | Professor |
8.Core Members
| Names | Institution,Department | Title of Position |
| Shibata, Shigenobu | Waseda University, School of Human Sciences | Professor |
9.Cooperating Researchers
| Names | Institution,Department | Title of Position |
| Ikeda, Masayuki | Waseda University, Advanced Research Institute for Science and Engineering | Lecturer |
| Hirono, Moritoshi | Waseda University, Advanced Research Institute for Science and Engineering | Assistant |
| Moriya, Tkahiro | Waseda University, Advanced Research Center for Human Sciences | Assistant |
10.Summary of Research Results
|
During the research period we have focused our interest into visualization of signaling pathway using several kinds of knock out mice, such as NR2A(-/-), NR2C(-/-), NR2AC(-/-), PLCβ4(-/-) and GFAP(-/-). Neuron of the mice we observed here are in the hippocampus, thalamus, cerebellum and amygdala, and we examined the kinetics of neuron by patch clump experiment, Ca imaging, in-site hybridizations, and in-gel-assay. Abnormal behaviors of the knock out mice were assayed by eye-blinks reflex conditioning, fear conditioning, circadian rhythm and sleep (slow wave sleep, rem sleep and awake). Protein molecules we managed in the experiment are PLCβ4 and voltage sensitive Ca channels. Therefore the research project we carried out in this period can be expressed briefly as Ca signaling in the neuron. We have found that PLCβ4 in the rostral part of the cerebellum are essential to form LTD and eye blink reflex conditioning and it is also very significant that PLCβ4 is localized at thalamus to make REM sleep. On the other hand PLCβ4 conditioned in supra chiasmatic nucleus (SCN) is not useful to regulate circadian rhythm. For the future development of the neuroscience we have established two kinds of novel technologies, which are thermal imaging and transfection of Ca sensitive fluorescent protein in the organella, such as ER, SR and Mitochondria. Thermal imaging reflects which proteins in the molecular circuit are involved in the desired signal transduction. Cameleon and Pericam, both of which are initially developed by Dr. Miyawaki, were transfected in the neuron as well as in the neuronal cell lines and successfully observed Ca correlation between cytosol and other kinds of organella. Using these newly developed techniques, every area of the neuroscience will be promoted largely in the near future. |
11.Key Words
(1)knock out mice、(2)Ca signaling、(3)Voltage dependent Ca channel
(4)Ca activated K channel、(5)Phospholipase C、(6)Associative learning
(7)Eye blinks reflex、(8)Circadian Rhythm、(9)Sleep
12.References
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