Summary of Research Project Results Under the JSPS FY2000
"Research for the future Program"



1.Research Institution Kyoto University
 
2.Research Area Integrated Fields
 
3.Research Field Tissue Engineering
 
4.Term of Project FY1996〜FY2000
 
5.Project Number 96I00204
 
6.Title of Project Tissue Engineering for Organ Regeneration

7.Projetct Leader
Name Institution,Department Title of Position
Yoshio Yamaoka Kyoto University Graduate School of Medicine Professor

8.Core Members

Names Institution,Department Title of Position
Kazutomo Inoue Kyoto University Institute of Frontier Medical Science Professor
Hiroo Iwata Kyoto University Institute of Frontier Medical Science Professor
Iwao Ikai Kyoto University Graduate School of Medicine Lecturer

9.Cooperating Researchers

Names Institution,Department Title of Position
Yoshihito Honda Kyoto University Graduate School of Medicine Professor
Kazuwa Nakao Kyoto University Graduate School of Medicine Professor
Masasi Komeda Kyoto University Graduate School of Medicine Professor

10.Summary of Research Results

The "Tissue Engineering for Organ Regeneration" project has progressed through cooperative studies in medicine and engineering. In the artificial liver study, we've developed a liver support system using ex-vivo perfused porcine liver. We confirmed the beneficial effects of the system using the hDAF transgenic porcine liver in a liver assist device in vivo. In 1997, however, it was reported that multiple proviruses of porcine endogenous retrovirus (PERV) are integrated into the genome of all pigs, and virus particles, some of which are able to infect human cells, are released from normal pig cells. To evaluate the potential risk posed by the transmission of PERVs from porcine liver in the liver support system, baboons cross-circulated with this system were examined. A transient increase of PERV in blood samples of the baboons was detected, so we stopped clinical use of the device using porcine liver. In addition, we have developed the hybrid bioartificial liver using porcine hepatocytes, but it also poses a risk for PERV transmission.
Therefore, we altered the source of hepatocytes for our bioartificial liver from porcine hepatocytes to human liver stem cells. We are now studying isolation techniques and characterization of these cells.
For the bioartificial pancreas, we also have studied isolation of xenogeneic islet cells, development of membranes as immunological barriers, convenience, safety issues, and suitable portions for transplantation. However, since it is difficult to use xenogeneic islets in a bioartificial pancreas, clinically, we are studying alternative sources of islet cells. In addition, we have advanced basic scientific knowledge in the use of organ stem cells in the regeneration of retina, heart, vessels, fatty tissues of breast, kidneys and the urinary bladder. We are now making an effort to develop human cells as a resource for clinical use instead of xenogeneic biomaterial.

11.Key Words

(1)Bioartificial Liver、(2)Bioartificial Pancreas、(3)Xenogeneic cells
(4)Human Stem Cell、(5)Immunoisolation、(6)Organ Regeneration

12.References

[Reference Articles]
Author Title of Article
T. Hirose Oxgen dependency of epidermal growth factor receptor binding and DNA synthesis of rat hepatocytes
Journal Volume Year Pages Concerned
Journal of Hepatology 27・ 1997 1081-1088

Author Title of Article
N. Shinkura Presence of anti-FKBP12 autoantibodies in patients with liver allografts
Journal Volume Year Pages Concerned
Transplantation 64・(9) 1997 1336-1342

Author Title of Article
T. Yagi The protective effects of prostaglandin E, on sinusoidal endothelial cells in xenogeneic pig liver perfusion
Journal Volume Year Pages Concerned
Journal of Surgical Research 73・(1) 1997 28-34

Author Title of Article
S. Satoh Humoral injury in porcine livers perfused with human whole blood.
Journal Volume Year Pages Concerned
Transplantation 64・(8) 1997 1117-1123

Author Title of Article
T. Yagi Extracorporeal xenoperfusion of the pig liver as a liver assist device:Effects of prostaglandin E, and soluble complement receptor type 1
Journal Volume Year Pages Concerned
Transplantation Proceedings 30・(1) 1998 74-75

Author Title of Article
K. Kinoshita Exposure of hepatic sinusoidal mononuclear cells to UW solution in situ but not ex vivo induces apoptosis.
Journal Volume Year Pages Concerned
Journal of Hepatology 29・(2) 1998 300-305

Author Title of Article
K. Yamagami Heat schok preconditioning ameliorates liver injury following normothermic ischemia-reperfusion in steatotic rat livers.
Journal Volume Year Pages Concerned
Journal of Surgical Research 79・(1) 1998 47-53

Author Title of Article
R. Terao Suppression of Proliferative Cholangitis in a Rat Model with Direct Adenovirus-Mediated Retinoblastoma Gene Transfer to the Biliary Tract.
Journal Volume Year Pages Concerned
Hepatology 28・(3) 1998 605-612

Author Title of Article
S. Tsuyuki N-acetylcysteine Improves Cytotoxic Activity of Cirrhotic Rat Liver-associated Mononuclear Cells
Journal Volume Year Pages Concerned
International Immunology 10・10 1998 1501-1508

Author Title of Article
S. Tsuyuki Possible Availability of N-Acetylcysteine as an Adjunct to Cytokine Therapy for Hepatocellular Carcinoma
Journal Volume Year Pages Concerned
Clinical Immunology and Immunopathology 88・2 1998 192-198

Author Title of Article
A. Kanazawa The Beneficial Effect of Phosphocreatine Accumulation in the Creatine Kinase Transgenic Mouse Liver in Endotoxin-Induced Hepatic Cell Death
Journal Volume Year Pages Concerned
Journal of Surgical Research 80・(2) 1998 229-235

Author Title of Article
N. Shinkura Autoantibodies to FK506 Binding Protein 12 (FKBP12) in Autoimmune Diseases
Journal Volume Year Pages Concerned
Autoimmunity 29・ 1999 159-170

Author Title of Article
Y. Kawai Hepatocyte growth inhibitory factor derived from HTLV-I(+) T-cell line is identical to IL-6
Journal Volume Year Pages Concerned
Leukemia Research 23・ 1999 489-497

Author Title of Article
T. Uehara Gene Trasfer to the Rat Biliary Tract with the HVJ-cationic Liposome Method
Journal Volume Year Pages Concerned
Journal of Hepatology 30・ 1999 836-842

Author Title of Article
T. Uesugi Evaluation of Ammonia and Lidocaine Clearance, and Galactose Elimination Capacity of Xenoperfused Pig Livers Using a Pharmacokinetic Analysis
Journal Volume Year Pages Concerned
Transplantation 68・(2) 1999 209-214

Author Title of Article
H. Nakayama Pharmacologic stimulation of adenosine A2 receptor supplants ischemic preconditioning in providing ischemic tolerance in rat livers
Journal Volume Year Pages Concerned
Surgery 126・5 1999 945-954

Author Title of Article
Y. Ishikawa Heat Shock Preconditioning on Mitochondria during Warm Ischemia in Rat Livers
Journal Volume Year Pages Concerned
Journal of Surgical Research 87・2 1999 178-184

Author Title of Article
S. Satoh ANXN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1,
Journal Volume Year Pages Concerned
Nature Genetics, 24 (3), 245-250 24・(3) 2000 245-250

Author Title of Article
S. Kimoto The augmentative effect of repeated heat shock preconditioning on the production of heat shock protein 72 and on ischemic tolerance in rat liver tissue,
Journal Volume Year Pages Concerned
International Journal of Hyperthermia 16・(3) 2000 247-261

Author Title of Article
K. Yamagami Effects of geranyl-geranyl-acetone administration before heat shock preconditioning for conferring tolerance against ischemia-reperfusion injury in rat livers,
Journal Volume Year Pages Concerned
J. Lab Clin Med 135・(6) 2000 465-475

Author Title of Article
I. Ikai Xenogeneic extracorporeal liver perfusion as a liver assist device,
Journal Volume Year Pages Concerned
Tissue Engineering for Therapeutic Use 4. (Y. Ikada and Y. Shimizu, editors. )   2000 177-188

Author Title of Article
H. Yamamoto Heat-shock preconditioning reduces oxidative protein denaturation and ameliorates liver injury by carbon tetrachloride in rats,
Journal Volume Year Pages Concerned
Research in Experimental Medicine 199・ 2000 309-318

Author Title of Article
M. Kume Pharmacological hepatic preconditioning:involovement of 70-kDa heat shock proteins (HSP72 and HSP73) in ischaemic tolerance after intravenous administration of doxorubicin
Journal Volume Year Pages Concerned
British Journal of Surgery 87・ 2000 1168-75

Author Title of Article
H. Okabe Comprehensive allelotype study of hepatocellular carcinoma: Potential differences in pathways to hepatocellular carcinoma between hepatitis B virus -Positive and- Negative tumors
Journal Volume Year Pages Concerned
Hepatology 31・5 2000 1073-1079

Author Title of Article
O. Takeyama, Effects of prostaglandin E1 on the efficacy of xenogeneic extracorporeal pig liver perfusion in a canine model of acute liver failture,
Journal Volume Year Pages Concerned
Liver Transplantation,   in press  

Author Title of Article
T. Uesugi Influences of Humoral Immunoreaction on Hepatic Non-parenchymal Cells in Ex Situ Xenoperfused Rat Livers1
Journal Volume Year Pages Concerned
Journal of Surgical Research(J Surg Res)   in press  

Author Title of Article
R. Nishitai Influence of Extracorporeal Porcine Liver Perfusion on Non-Human Primate: Minimizing Hemolysis Improves Subsequent Survival.
Journal Volume Year Pages Concerned
Liver Transplantaion   in press  

Author Title of Article
H. Kishimoto Clinical trial of prophylactic endscopic variceal ligation for esophageal varices
Journal Volume Year Pages Concerned
Journal of Gasrtoenterology 32・ 1997 6-11

Author Title of Article
H. Kaii The effect of MX-1on survival of discordant cardiac xenograft from guinea pig to rat.
Journal Volume Year Pages Concerned
Transplant Immunology 5・ 1997 70-72

Author Title of Article
M. Inai Endosonographic characterzation of duodenal elevated leisons.
Journal Volume Year Pages Concerned
Gastrointestinal Endoscopy 44・ 1997 714-719

Author Title of Article
Y. Kawakami Subcutaneous xenotransplantation of hydro bioartificial pancreas encapsulating pancreatic B-cell line (MIN6).
Journal Volume Year Pages Concerned
Cell Transplantation 6・ 1997 541-545

Author Title of Article
Y. Kawakami Prolonged effect of troglitazone (CS-045) on xenograft survival of hybrid artificial pancreas.
Journal Volume Year Pages Concerned
Cell Transplantation 6・ 1997 547-550

Author Title of Article
H. Kaji The effect of MX-1 and FOY on survival of discordant cardiac xenograft.
Journal Volume Year Pages Concerned
Transplantation Proceedings 29・ 1997 3024-3026

Author Title of Article
T. Yamasaki Effect of a new immunosuppressive agent, FTY720, on survival of islet allografts
Journal Volume Year Pages Concerned
Cell Transplantation 7・(4) 1998 403-406

Author Title of Article
Y. J. Gu Improvement of Adult Porcine Pancreatic Islet Isolation; Employment of an Innovative Enzyme Solution
Journal Volume Year Pages Concerned
Transplantation Proceedings 30・ 1998 356-357

Author Title of Article
H. Setoyama The Potential of Anticomplement Synthetic Sulfonic Polymers for Xenotransplantation
Journal Volume Year Pages Concerned
Transplantation Proceedings 30・ 1998 67-70

Author Title of Article
M. Miyamoto Improvement of a large-scale isolation procedure for breeder porcine islets. -Possibility of harvesting from non-heart beating donor-
Journal Volume Year Pages Concerned
Cell transplantation 7・ 1998 397-402

Author Title of Article
M. Miyamoto Effect of "acidic oxidative potential water" on mivrobial contamination harvesting porcine panvreas for islet xenotransplantation.
Journal Volume Year Pages Concerned
Transplant Proc 30・ 1998 3431-3432

Author Title of Article
M. Miyamoto Establishment of Fluorescein Diacetate and Ethidium Bromide (FDAEB) Assay for Isolation Islets in Quality Assessment of Islets.
Journal Volume Year Pages Concerned
Cell Transplantation 9・ 2000 681-686

Author Title of Article
M. Miyamoto Effectiveness of Acidic Oxidative Potential Water in Preventing Bacterial in Islet Transplantation.
Journal Volume Year Pages Concerned
Cell Transplantation 8・ 1999 405-411

Author Title of Article
Cui W. Novel Method for Isolation of Adult Porcine Pancreas Islet with Two-Stage Digestion Procedure
Journal Volume Year Pages Concerned
Cell Transplantation 8・ 1999 391-398

Author Title of Article
H. Suwa Detection of multiple hepatic micrometastases in pancreatic adenocarcinoma with a solitary liver metastasis by direct sequencing of the K-ras gene
Journal Volume Year Pages Concerned
A case report, Surgery 124・ 1999 113-116

Author Title of Article
M. Miyamoto Effectiveness of modified two layer preservation method (PEC/Kyotosolution) in Islet Isolation of breeder pigs
Journal Volume Year Pages Concerned
Transplant Proc 32・ 2000 1660-1661

Author Title of Article
K. Inoue Basic knowledge of interest- Islet transplantation,
Journal Volume Year Pages Concerned
Journal of Hepatobiliary Pancreatic Surgery 7・ 2000 163-177

Author Title of Article
K. Inoue Invited Critiques for"Laparoscopy-Assisted Billroth I Gastrectomy Compared with Conventional Open Gastrectomy"
Journal Volume Year Pages Concerned
, Archives of Surgery 135・7 2000 810

Author Title of Article
Y. J. Gu Effect of Neovascularization-inducing Bioartificial Pancreas on Survival of Syngeneic Islet Graft
Journal Volume Year Pages Concerned
Transplant Proc 32・ 2000 2494-2495

Author Title of Article
Y. J. Gu DEVELOPMENT OF A NEW BIO-ARTIFICIAL PANCREAS POSSESSING ANGIOGENESIS-INDUCING FUNCTION
Journal Volume Year Pages Concerned
Transplant Proc 32・ 2000 2475

Author Title of Article
Y. Kawakami Modified subcutaneous tissue with nevascularization as the site for pancreatic islet ransplantation
Journal Volume Year Pages Concerned
Cell Transplantation   in press  

Author Title of Article
N. Kinoshita Regulation of Cell Proliferation using Tissue Engineering in MIN6 cells
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
M. Miyamoto Development of cryopreservation procedure of freezer bag for pancreatic islets using newly developed cryoprotectant
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
Wenjing Wang Effect of Basic Fibroblast Growth Factor on Insulin Secretin From Micronecapsulated Pancreatic Islets; An in vitro study
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
H. Hori Isolation, Culture and Characterization of Endocrine Cells from Six-months-old Porcine Pancreas
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
Y. J. Gu. Development of a new method to induce angiogenesis at subcutaneous site of strepotozotocin-induced diabetic rats for islet transplantation
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
Wanxing Cui. The Tissue-engineering pancreatic Islets ミ Culturing The Rat Islets in The Chitosan Sponge
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
N. Nagata Evaluation of Insulin Secretion of Isolated Rat Islets Cultured in Extracellilar Matrix
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
A. N. Balamuruga Prevascularized Intermuscular Space: A Potential Site for Bioartficial Pancreas Transplantation
Journal Volume Year Pages Concerned
Cell transplantation   in press  

Author Title of Article
Baoyou Xu The influence of anticomplement synthetic sulfonic polymers on function of pancreatic islets : an in vitro study
Journal Volume Year Pages Concerned
Cell Transplantation   in press  

Author Title of Article
Baoyou Xu Functional comparison of the Agarose microbeads and the developed three layer Agarose microbeads as the Bioartificial pancreas : An in vitro study
Journal Volume Year Pages Concerned
Cell Transplantation   in press  

Author Title of Article
H. Iwata Hybrid Artificial Organs. Polymeric Materials Encyclopedia
Journal Volume Year Pages Concerned
J. C. Salamone Ed. ?.? 1996 3082-3087

Author Title of Article
H. Iwata Permeability of filters used for immunoisolation.
Journal Volume Year Pages Concerned
Tissue Engineering 2・ 1996 289-298

Author Title of Article
H. Iwata Atomic Force Microscopic Images of Solvated Polymer Brushes
Journal Volume Year Pages Concerned
Langmuir 13・ 1997 3063-3066

Author Title of Article
K. Kazekawa Nontoxic Embolic Liquids for Treatment of Arteriovenous Malformations.
Journal Volume Year Pages Concerned
J. Biomed. Mater. Res. (Appl. Biomater. ), 38・ 1997 79-86

Author Title of Article
K. Yamada Development of a Dural Substitute from Synthetic Bioabsorbable Polymers.
Journal Volume Year Pages Concerned
J. Neurosurg 86・ 1997 1012-1017

Author Title of Article
T. Samejima Gelatin density gradient for isolation of islets of Langerhans
Journal Volume Year Pages Concerned
Cell Transplantation 7・ 1998 37-45

Author Title of Article
H. Noguchi Synthesis of monomeric and polymeric conjugates carrying a thrombin Inhibitor through an ester bond.
Journal Volume Year Pages Concerned
J. Biomed. Mater. Res 39・ 1998 621-629

Author Title of Article
N. Hisano Entrapment of islets into reversible disulfide hydrogels.
Journal Volume Year Pages Concerned
J. Biomed. Mater. Res. 40・ 1998 115-123

Author Title of Article
H. Iwata Atomic force microscopic analysis of a porous membrane with pH-sensitive molecular valves
Journal Volume Year Pages Concerned
Macromolecules 31・ 1998 3671-3678

Author Title of Article
V. N. Vasilets Photolysis of a fluorinated polymer film by vacuum ultraviolet radiation.
Journal Volume Year Pages Concerned
J. Polym. Sci. A: Polym. Chem. 36・ 1998 2215-2222

Author Title of Article
Y. G. Park Microencapsulation of islets and model beads with thin alginate-Ba+ gel layer by centrifugation
Journal Volume Year Pages Concerned
Polym Adv Technol 9・ 1998 734-739

Author Title of Article
H. Iwata A novel surgical glue composed of gelatin and N-hydroxysuccinimide activated poly(L-glutamic acid): Part 1. Synthesis of activated poly(L-glutamic acid) and its gelation with gelatin
Journal Volume Year Pages Concerned
Biomaterials 19 1998 1869-1876

Author Title of Article
H. Iwata A Mode for oxygen transport in microencapsulated islets
Journal Volume Year Pages Concerned
MRS Symposium Proceedings 530・ 1998 19-24

Author Title of Article
H. Iwata Importance of the Extracorporeal Circulation Rate in a Bioartificial Liver
Journal Volume Year Pages Concerned
Materials Science and Engineering C6・ 1998 235-243

Author Title of Article
B. Zhu Preparation of DNA-immobilized immunoadsorbent for treatment of systemic lupus erthematosus.
Journal Volume Year Pages Concerned
J. Biomater. Sci. Polymer Edn ? 1999 341-350

Author Title of Article
S. Matsuda Bioadhesion of gelatin filmas crosslinked withg glutaraldehyde
Journal Volume Year Pages Concerned
J. Biomed. Mater. Res. 45・ 1999 20-27

Author Title of Article
H. Iwata Control of complerment activities for immunoisolation
Journal Volume Year Pages Concerned
Bioartigicial Orangs II 875・ 1999 7-23

Author Title of Article
H. Iwata In vitro evaluation of metabolic functions of a bioartificial liver.
Journal Volume Year Pages Concerned
ASAIO J. 45・ 1999 299-306

Author Title of Article
H. Iwata Agarose. Cell Encapsulation Technology and Therapeutics, W. M. Kutreiber, R. P. Lanza, and W. L. Chick, eds
Journal Volume Year Pages Concerned
Birkhaser 45 1999 97-107

Author Title of Article
B. Zhu Immobilization of poly (ethelene imine) into polymer films pretreated with plasma
Journal Volume Year Pages Concerned
J. Appl. Polym. Sci 75 2000 576-582

Author Title of Article
B. Zhu Hydrophilic modifidation of a polyimide film surface
Journal Volume Year Pages Concerned
J. Adhesion Sci. Technol. 14・(3) 2000 351-361

Author Title of Article
T. Sajiki Morphologic studies of hepatocytes entrapped in hollow fibers of a bioartificial liver
Journal Volume Year Pages Concerned
ASAIO J. 46・(1) 2000 49-55

Author Title of Article
E. Uchida Surface structure of poly (ethylene terephthalate) film grafted with poly (methacrylic acid)
Journal Volume Year Pages Concerned
Polymer 41・ 2000 3609-3614

Author Title of Article
H. Oowaki, Non-adhesive cyanoacrylate as an embolic material for endovascular neurosurgery.
Journal Volume Year Pages Concerned
Biomaterials 21・ 2000 1039-1046

Author Title of Article
T. Richey Surface modification of polyethylene balloon catheters for local drug delivery.
Journal Volume Year Pages Concerned
Biomaterials 21・ 2000 1057-1065

Author Title of Article
X. Mo Soft tissue adhesive composed of modified gelatin and polysaccharides
Journal Volume Year Pages Concerned
J. Biomater. Sci Polymer Edn 11・ 2000 341-351

Author Title of Article
I. Hirata Study of complement activation on well-defined surfaces using surface plasmon resonance, Colloids and Surfaces B
Journal Volume Year Pages Concerned
Biointerfaces 18・ 2000 285-292

Author Title of Article
T. Sajiki Transmission electron microscopic study of hepatocytes in bioartificial liver
Journal Volume Year Pages Concerned
TISSUE ENGINEERING 6 2000 627-640

Author Title of Article
I. Hirata Surface modification of Si3N4-coated silicon plate for investigation of living cells.
Journal Volume Year Pages Concerned
Jpn. J. Appl. Phys. 39・ 2000 6441-6442

Author Title of Article
E. Itoh Synthetic absorbable film for prevention of air leaks after stapled pulmonary resection
Journal Volume Year Pages Concerned
J. Biomed. Mater. Res. (Appl. Biomater. ) 53・ 2000 640-645

Author Title of Article
Seigel GM, Intraocular transplantation of E1A-immortalized retinal precursor cells.
Journal Volume Year Pages Concerned
Cell Transplant 7・ 1998 559-566

Author Title of Article
M. Takahashi Wide spread integration and survival of adult-derived neural progenitor cells in the developing optic retina
Journal Volume Year Pages Concerned
Mol Cell Neurosci 12・ 1998 340-348

Author Title of Article
A. Nishida Incorporation and differentiation of hippocampus-derived neural stem cells transplanted in injured adult rat retina.
Journal Volume Year Pages Concerned
Invest Ophthalmol Vis Sci 41・ 2000 4268-4274

Author Title of Article
M. Mandai Long-Term Outcome After Radiation Therapy for Subfoveal Choroidal Neovascularization Associated with Age-Related Macular Degeneration.
Journal Volume Year Pages Concerned
Jpn J Ophthalmol. 44・ 2000 530-537

Author Title of Article
M. Harada Significance of ventricular myocytes and nonmyocytes interaction during cardiocyte hypertrophy. -evidence for endothelin-1 as a paracrine hypertrophic factor from cardiac nonmyocytes-
Journal Volume Year Pages Concerned
Circulation 96・ 1977 3737-3744

Author Title of Article
K. Kuwahara Endothelin-1 and cardiotrophin-1 induce brain natriuretic peptide gene expression by distinct transcriptional mechanisms
Journal Volume Year Pages Concerned
J. Cardiovas. Pharmacol. 31・? 1998 s354-s356

Author Title of Article
A. Yasoda Natriuretic peptide regulation of endochondral ossification: Evidence for possible roles of C-type natriuretic peptide/guanylyl cyclase-B pathway.
Journal Volume Year Pages Concerned
J. Biol. Chem. 273・ 1998 11695-11700

Author Title of Article
M. Suda Skeletal overgrowth in transgenic mice that overexpress brain natriuretic peptide.
Journal Volume Year Pages Concerned
Proc. Natl. Acad. Sci. USA 95・ 1998 2337-2342,

Author Title of Article
H. Itoh, Hypertension and insulin resistance: role of peroxisome proliferator-activated receptor gamma
Journal Volume Year Pages Concerned
Clin-Exp-Pharmacol-Physiol 26・(7) 1999 558-560

Author Title of Article
H. Itoh Vascular stress response and endothelial vasoactive factors for vascular remodeling.
Journal Volume Year Pages Concerned
Diabetes Res. Clin. Pr. 45・ 1999 83-88

Author Title of Article
M. Ishikawa A Heart-specific increase in cardiotrophin-1 gene Expression precedes establishment of ventricular hypertension in genetically hypertensive rats.
Journal Volume Year Pages Concerned
J. Hypertens 17・(6) 1999 807-816

Author Title of Article
K. Kuwahara The effects of the selective ROCK inhibitor, Y27632, on ET-1-induced hypertrophic response in neonatal rat cardiac myocytes--possible involvement of Rho/ROCK pathway in cardiac muscle cell hypertrophy
Journal Volume Year Pages Concerned
FEBS-Lett 452・(3) 1999 314-318

Author Title of Article
K. Kuwahara Involvement of cardiotrophin-1 in cardiac myocyte-nonmyocyte interactions during hypertrophy of rat cardiac myocytes in vitro
Journal Volume Year Pages Concerned
Circulation 100・ 1999 1116-1124

Author Title of Article
N. Sawada Inhibition of Rho-associated kinase results in suppression of neointimal formation of balloon-injured arteries
Journal Volume Year Pages Concerned
Circulation 101・ 2000 2030-2033

Author Title of Article
Y. Fukunaga Altered gene expression of uncoupling protein-2 and -3 in stroke-prone spontaneously hypertensive rats
Journal Volume Year Pages Concerned
J. Hypertens 18・ 2000 1233-1238

Author Title of Article
I. Hamanaka Effects of Cardiotrophin-1 on Hemodynamics and Endocrine Function of the Heart.
Journal Volume Year Pages Concerned
Am. J. Physiol. 279・(1) 2000 H388-396

Author Title of Article
N. Tamura Cardiac fibrosis in mice lacking brain natriuretic peptide. Proc.
Journal Volume Year Pages Concerned
Natl. Acad. Sci. USA. 97・(8) 2000 4239-4244

Author Title of Article
E. Ogawa Outside-in Signaling of Fibronectin Stimulates Cardiomyocyte Hypertrophy in Cultured Neonatal Rat Ventricular Myocytes
Journal Volume Year Pages Concerned
J. Mol. Cell. Cardiaol. 32・(5) 2000 765-776

Author Title of Article
Y. Miyamoto Replication protein Al reduces transcription of the endothelial nitric oxide synthase gene containing a T-786→C mutation associated with coronary spastic angina.
Journal Volume Year Pages Concerned
Human Molecular Genetics 9・(18) 2000 2629-2637

Author Title of Article
S. Yura Accelerated puberty and late-onset hypothalamic hypogonadism in female transgenic skinny mice overexpressing leptin.
Journal Volume Year Pages Concerned
J. Clin. Invest. 105・ 2000 749-755

Author Title of Article
M. Aizawa-Abe Pathophysiological role of leptin in obesity-related hypertension.
Journal Volume Year Pages Concerned
J. Clin. Invest. 105・ 2000 1243-1252

Author Title of Article
H. Chusho Genetic models reveal that brain natriuretic peptide can signal through different tissue-specific receptor-mediated pathways.
Journal Volume Year Pages Concerned
Endocrinology 141・ 2000 3807-3813

Author Title of Article
N. Sawada cGMP-dependent protein kinase phosphorylated and inactivates RhoA.
Journal Volume Year Pages Concerned
Biochem. Biophys. Res. Commun. 280・ 2001 798-805

Author Title of Article
K. Kuwahara Neuron-Restrictive Silencer Element/Neuron-Restrictive Silencer Factor System Regulates Basal-and Endothelin-1-Inducible Atrial Natriuretic Peptide Gene Expression in Ventricular Myocytes
Journal Volume Year Pages Concerned
Mol Cell Biol. 21・ 2001 2085-2097

Author Title of Article
M. Inoue Oxidized low density lipoprotein regulates VEGF expression in human macrophages and endothelial cells through activation of PPAR g.
Journal Volume Year Pages Concerned
Arterioscler. Thromb. Vasc. Biol   in press  

Author Title of Article
K. Doi C-type natriuretic peptide induces re-differentiation of vascular smooth muscle cells with accelerated re-endothelialization
Journal Volume Year Pages Concerned
Arterioscler. Thromb. Vasc. Biol   in press  

Author Title of Article
I. Hamanaka Induction of JAB/SOCS-1/SSI-1 and CIS3/SOCS3/SSI-3 Is Involved in gp130 Resistance in Cardiovascular System in Rat Treated with Cardiotrophin-1 (CT-1) in vivo.
Journal Volume Year Pages Concerned
Circ. Res   in press  

Author Title of Article
H. Chusho Dwarfism and early death in mice lacking C-type natriuretic peptide.
Journal Volume Year Pages Concerned
Proc. Natl. Acad. USA   in press  

Author Title of Article
Y. Tabata De novo formation of adipose tissue by controlled release of basic fibroblast growth factor.
Journal Volume Year Pages Concerned
Tissue Engineering 6・(3) 2000 279-289

[Reference Books]
Author Title of Book
Y. Ikada, editors Y. Yamaoka, Co-editor Tissue Engineering for Therapeutic Use 1
Publisher Year Pages
Elsevier Science 1998 159

Author Title of Book
Y. Ikada, editors S. Enomoto, Co-editor Tissue Engineering for Therapeutic Use 2
Publisher Year Pages
Elsevier Science 1998 137

Author Title of Book
Y. Ikada, editors T. Okano, Co-editor Tissue Engineering for Therapeutic Use 3
Publisher Year Pages
Elsevier Science 1999 161

Author Title of Book
Y. Ikada, editors Y. Shimizu, Co-editor Tissue Engineering for Therapeutic Use 4
Publisher Year Pages
Elsevier Science 2000 192

Author Title of Book
Y. Ikada, editors N. Ohshima, Co-editor Tissue Engineering for Therapeutic Use 5
Publisher Year Pages
Elsevier Science 2001 226


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