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Experiences and Message from JSPS Fellows

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Dr. Appolinaire Adandonon


Profile

Nationality

Benin

Host institute

National Institute of Agro-Environmental Sciences

Host researcher

Dr. Ikuko OKABE

Fellowship period

2005/11/30 2007/11/29

Research field

Biological Sciences

photo

Research title

Microbial Community on Sclerotia of Sclerotium Rolfsii

 

The reason for choosing Japan to conduct research

My wishes after my PhD were to get PostDoc research in a none-African country. Fortunately, I heard about the JSPS scholarship and because Japan is a high-standard-level-technology Country, my dream was to get the fellowship. During my PhD studies, I have been in email-contact with Dr Ikuko Okabe working in a similar research field. So together with her, as Host Researcher, I applied for and got the fellowship and came to Japan.

Research achievements

As objective, study effects of flooding on microorganisms associated with sclerotia debilitation. 16S-rDNA-PCR-DGGE patterns and sequence analysis were used on sclerotia sample from greenhouse and field. Without flooding, Oxalobacteraceae, Nocardiaceae and Actinomycetaceae were dominant, whereas under flooding, major bacterial groups were Clostridiaceae. In another fluorescent in situ hybridization (FISH) study, Cy3-labeled oligonucleotide probe (S-G-Pan-0059-a-A-18) was shown to be promising for studying E. herbicola and E. ananas population (published). Next is to clarify roles of bacterial communities in fungistasis, leading to sclerotia debilitation and trace temporal changes of bacterial population on sclerotia using the FISH-based oligonucleotide probe.

Advice to new fellows

As advice: take Japanese language class; make friends; take active part in social activities; try JSPS-Science-Dialogue program. For within-Japan travel, use the following website for itinerary:
http://v202.jorudan.co.jp/norikae/cgi-bin/engkeyin.cgi?proc_sw=0